Description
Antibody Name: | Anti-KIF5B Antibody (CAB20928) |
Antibody SKU: | CAB20928 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunofluorescence |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human KIF5B. |
Application: | Western blotting, Immunofluorescence |
Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | 293T, HepG2, MCF7, Mouse lung, Rat skeletal muscle, Mouse brain, Rat brain, Rat heart |
Immunogen: | Recombinant protein of Human KIF5B. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, cytoskeleton |
Calculated MW: | 109kDa |
Observed MW: | 120KDa |
Synonyms: | KIF5B, HEL-S-61, KINH, KNS, KNS1, UKHC |
Background: |
Immunofluorescence analysis of HeLa cells using KIF5B Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining. | |
Western blot analysis of extracts of various cell lines, using KIF5B antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. |