Anti-KIF5B Antibody (CAB15284)

Name: Anti-KIF5B Antibody (CAB15284)
Brand: Cell Biology Antibodies 5
SKU: CAB15284
Availability: OutOfStock

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OverviewAntibody Name:Anti-KIF5B Antibody (CAB20928)Antibody SKU:CAB20928Antibody Size:50µL, 100µLApplication:Western blotting, Immunofluorescence</

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Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HeLa, HepG2, Mouse brain, Mouse heart, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 650-770 of human KIF5B (NP_004512.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LQNV EQKK RQLE ESVD ALSE ELVQ LRAQ EKVH EMEK EHLN KVQT ANEV KQAV EQQI QSHR ETHQ KQIS SLRD EVEA KAKL ITDL QDQN QKMM LEQE RLRV EHEK LKAT DQEK SRKL HELT V
Gene ID:	3799
Uniprot:	P33176
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	109kDa
Observed MW:	110kDa

UniProt Protein Function:	KIF5B: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Belongs to the kinesin-like protein family. Kinesin subfamily.
UniProt Protein Details:	Protein type:Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 10p11.22 Cellular Component: cytoplasm; kinesin complex; membrane; microtubule organizing center; perinuclear region of cytoplasm; vesicle Molecular Function:microtubule binding; microtubule motor activity; plus-end-directed microtubule motor activity; protein binding Biological Process: axon guidance; cytoskeleton-dependent intracellular transport; microtubule-based movement; positive regulation of potassium ion transport; regulation of membrane potential; vesicle transport along microtubule
UniProt Code:	P33176
NCBI GenInfo Identifier:	417216
NCBI Gene ID:	3799
NCBI Accession:	P33176.1
UniProt Secondary Accession:	P33176,Q5VZ85, A0AVB2,
UniProt Related Accession:	P33176
Molecular Weight:	109,685 Da
NCBI Full Name:	Kinesin-1 heavy chain
NCBI Synonym Full Names:	kinesin family member 5B
NCBI Official Symbol:	KIF5B
NCBI Official Synonym Symbols:	KNS; KINH; KNS1; UKHC; HEL-S-61
NCBI Protein Information:	kinesin-1 heavy chain
UniProt Protein Name:	Kinesin-1 heavy chain
UniProt Synonym Protein Names:	Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; UKHC
Protein Family:	Kinesin
UniProt Gene Name:	KIF5B
UniProt Entry Name:	KINH_HUMAN

	Western blot analysis of extracts of various cell lines, using KIF5B antibody (CAB15284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse lung using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens).

Synonyms:	KIF5B, HEL-S-61, KINH, KNS, KNS1, UKHC
Background: