Cell Biology Antibodies 5
Anti-KIF5B Antibody (CAB15284)
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- SKU:
- CAB15284
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-KIF5B Antibody |
| Antibody SKU: | CAB15284 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 650-770 of human KIF5B (NP_004512.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, HeLa, HepG2, Mouse brain, Mouse heart, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 650-770 of human KIF5B (NP_004512.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LQNV EQKK RQLE ESVD ALSE ELVQ LRAQ EKVH EMEK EHLN KVQT ANEV KQAV EQQI QSHR ETHQ KQIS SLRD EVEA KAKL ITDL QDQN QKMM LEQE RLRV EHEK LKAT DQEK SRKL HELT V |
| Gene ID: | 3799 |
| Uniprot: | P33176 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 109kDa |
| Observed MW: | 110kDa |
| Synonyms: | KIF5B, HEL-S-61, KINH, KNS, KNS1, UKHC |
| Background: |
| UniProt Protein Function: | KIF5B: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Belongs to the kinesin-like protein family. Kinesin subfamily. |
| UniProt Protein Details: | Protein type:Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 10p11.22 Cellular Component: cytoplasm; kinesin complex; membrane; microtubule organizing center; perinuclear region of cytoplasm; vesicle Molecular Function:microtubule binding; microtubule motor activity; plus-end-directed microtubule motor activity; protein binding Biological Process: axon guidance; cytoskeleton-dependent intracellular transport; microtubule-based movement; positive regulation of potassium ion transport; regulation of membrane potential; vesicle transport along microtubule |
| UniProt Code: | P33176 |
| NCBI GenInfo Identifier: | 417216 |
| NCBI Gene ID: | 3799 |
| NCBI Accession: | P33176.1 |
| UniProt Secondary Accession: | P33176,Q5VZ85, A0AVB2, |
| UniProt Related Accession: | P33176 |
| Molecular Weight: | 109,685 Da |
| NCBI Full Name: | Kinesin-1 heavy chain |
| NCBI Synonym Full Names: | kinesin family member 5B |
| NCBI Official Symbol: | KIF5B |
| NCBI Official Synonym Symbols: | KNS; KINH; KNS1; UKHC; HEL-S-61 |
| NCBI Protein Information: | kinesin-1 heavy chain |
| UniProt Protein Name: | Kinesin-1 heavy chain |
| UniProt Synonym Protein Names: | Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; UKHC |
| Protein Family: | Kinesin |
| UniProt Gene Name: | KIF5B |
| UniProt Entry Name: | KINH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KIF5B antibody (CAB15284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse pancreas using KIF5B antibody (CAB15284) at dilution of 1:200 (40x lens). |
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