Cell Biology Antibodies 8

Anti-KIF5A Antibody (CAB3303)

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SKU:
CAB3303
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KIF5A Antibody
Antibody SKU:CAB3303
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 933-1032 of human KIF5A (NP_004975.2).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 933-1032 of human KIF5A (NP_004975.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TNPY GTRS PECI SYTN SLFQ NYQN LYLQ ATPS STSD MYFA NSCT SSGA TSSG GPLA SYQK ANMD NGNA TDIN DNRS DLPC GYEA EDQA KLFP LHQE TAAS
Gene ID:3798
Uniprot:Q12840
Cellular Location:Cytoplasm, cytoskeleton, perinuclear region
Calculated MW:117kDa
Observed MW:129kDa
Synonyms:KIF5A, D12S1889, MY050, NEIMY, NKHC, SPG10
Background:This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.
UniProt Protein Function:KIF5A: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the kinesin-like protein family. Kinesin subfamily.
UniProt Protein Details:

Protein type:Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: kinesin complex; microtubule; membrane; perinuclear region of cytoplasm; cytosol

Molecular Function:protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding

Biological Process: synaptic transmission; axon guidance; protein localization; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytoskeleton-dependent intracellular transport; blood coagulation; microtubule-based movement

Disease: Spastic Paraplegia 10, Autosomal Dominant

NCBI Summary:This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]
UniProt Code:Q12840
NCBI GenInfo Identifier:143811412
NCBI Gene ID:3798
NCBI Accession:Q12840.2
UniProt Secondary Accession:Q12840,Q4LE26, A6H8M5,
UniProt Related Accession:Q12840
Molecular Weight:117,378 Da
NCBI Full Name:Kinesin heavy chain isoform 5A
NCBI Synonym Full Names:kinesin family member 5A
NCBI Official Symbol:KIF5A  
NCBI Official Synonym Symbols:NKHC; MY050; SPG10; D12S1889  
NCBI Protein Information:kinesin heavy chain isoform 5A; KIF5A variant protein; neuronal kinesin heavy chain; kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific
UniProt Protein Name:Kinesin heavy chain isoform 5A
UniProt Synonym Protein Names:Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain; NKHC
Protein Family:Kinesin
UniProt Gene Name:KIF5A  
UniProt Entry Name:KIF5A_HUMAN
Anti-KIF5A Antibody (CAB3303)Western blot analysis of extracts of various cell lines, using KIF5A antibody (CAB3303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-KIF5A Antibody (CAB3303)Immunohistochemistry of paraffin-embedded mouse brain using KIF5A antibody (CAB3303) at dilution of 1:100 (40x lens).
Anti-KIF5A Antibody (CAB3303)Immunofluorescence analysis of PC-12 cells using KIF5A Rabbit pAb (CAB3303) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
Anti-KIF5A Antibody (CAB3303)Immunofluorescence analysis of SH-SY5Y cells using KIF5A Rabbit pAb (CAB3303) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
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