Antibody Name:	Anti-KIF4A Antibody
Antibody SKU:	CAB10193
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 870-1080 of human KIF4A (NP_036442.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, 293T, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 870-1080 of human KIF4A (NP_036442.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LIGE LVSS KIQV SKLE SSLK QSKT SCAD MQKM LFEE RNHF AEIE TELQ AELV RMEQ QHQE KVLY LLSQ LQQS QMAE KQLE ESVS EKEQ QLLS TLKC QDEE LEKM REVC EQNQ QLLR ENEI IKQK LTLL QVAS RQKH LPKD TLLS PDSS FEYV PPKP KPSR VKEK FLEQ SMDI EDLK YCSE HSVN EHED GDGD DDEG DDEE WKPT KLVK VSR
Gene ID:	24137
Uniprot:	O95239
Cellular Location:	Chromosome, Cytoplasm, Midbody, Nucleus matrix, cytoskeleton, spindle
Calculated MW:	128kDa/139kDa
Observed MW:	140kDa

Synonyms:

KIF4A, KIF4, KIF4G1, MRX100

Background:

This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

UniProt Protein Function:	KIF4A: Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. Belongs to the kinesin-like protein family. Chromokinesin subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Microtubule-binding; Motor Chromosomal Location of Human Ortholog: Xq13.1 Cellular Component: cytoplasm; cytosol; intercellular bridge; kinesin complex; membrane; midbody; nucleoplasm; spindle microtubule Molecular Function:microtubule motor activity; plus-end-directed microtubule motor activity; protein binding Biological Process: anterograde axon cargo transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytokinesis after mitosis; microtubule-based movement; organelle organization and biogenesis; retrograde vesicle-mediated transport, Golgi to ER; spindle midzone assembly involved in mitosis Disease: Mental Retardation, X-linked 100
NCBI Summary:	This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010]
UniProt Code:	O95239
NCBI GenInfo Identifier:	62512161
NCBI Gene ID:	24137
NCBI Accession:	O95239.3
UniProt Secondary Accession:	O95239,Q86TN3, Q86XX7, Q9NNY6, Q9NY24, Q9UMW3, B2R7V5 D3DVU4,
UniProt Related Accession:	O95239
Molecular Weight:	140kDa
NCBI Full Name:	Chromosome-associated kinesin KIF4A
NCBI Synonym Full Names:	kinesin family member 4A
NCBI Official Symbol:	KIF4A
NCBI Official Synonym Symbols:	KIF4; KIF4G1; MRX100
NCBI Protein Information:	chromosome-associated kinesin KIF4A
UniProt Protein Name:	Chromosome-associated kinesin KIF4A
UniProt Synonym Protein Names:	Chromokinesin-A
Protein Family:	Chromosome-associated kinesin
UniProt Gene Name:	KIF4A

	Western blot analysis of extracts of various cell lines, using KIF4A antibody (CAB10193) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat heart using KIF4A antibody (CAB10193) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using KIF4A antibody (CAB10193) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using KIF4A antibody (CAB10193) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using KIF4A antibody (CAB10193) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.