Cell Biology Antibodies 10

Anti-KIF3A Antibody (CAB6639)

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SKU:
CAB6639
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KIF3A Antibody
Antibody SKU:CAB6639
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF-7, SW480, 293T, SKOV3, HeLa, Mouse brain, Mouse ovary, Rat liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RKAL ETKL DMEE EERN KARA ELEK REKD LLKA QQEH QSLL EKLS ALEK KVIV GGVD LLAK AEEQ EKLL EESN MELE ERRK RAEQ LRRE LEEK EQER LDIE EKYT SLQE EAQG KTKK LKKV WTML MAAK SEMA DLQQ EHQR EIEG LLEN IRQL SREL RLQM LIID NFIP RDYQ EMIE NYVH WNED IGEW QLKC VAYT GNNM RKQT PVPD KKEK DPFE VDLS HVYL AYTE ESLR QSLM KLER PRTS KGKA RPKT GRRK RSAK PETV IDSL LQ
Gene ID:11127
Uniprot:Q9Y496
Cellular Location:Cell projection, Cytoplasm, cilium, cytoskeleton
Calculated MW:80kDa
Observed MW:80kDa
Synonyms:KIF3A, FLA10, KLP-20
Background:
UniProt Protein Function:KIF3A: Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Heterodimer of KIF3A and KIF3B. Interacts with PIFO/C1orf88. Interacts with CLN3. Belongs to the kinesin-like protein family. Kinesin II subfamily.
UniProt Protein Details:

Protein type:Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: centrosome; cytosol; kinesin II complex; microtubule cytoskeleton; spindle microtubule

Molecular Function:plus-end-directed microtubule motor activity; protein binding; spectrin binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cilium biogenesis; microtubule-based movement; neurogenesis; organelle organization and biogenesis; retrograde vesicle-mediated transport, Golgi to ER

UniProt Code:Q9Y496
NCBI GenInfo Identifier:296439481
NCBI Gene ID:11127
NCBI Accession:Q9Y496.4
UniProt Secondary Accession:Q9Y496,Q59EN1, Q86XE9, Q9Y6V4, A8MSW9,
UniProt Related Accession:Q9Y496
Molecular Weight:80,041 Da
NCBI Full Name:Kinesin-like protein KIF3A
NCBI Synonym Full Names:kinesin family member 3A
NCBI Official Symbol:KIF3A  
NCBI Official Synonym Symbols:FLA10; KLP-20  
NCBI Protein Information:kinesin-like protein KIF3A
UniProt Protein Name:Kinesin-like protein KIF3A
UniProt Synonym Protein Names:Microtubule plus end-directed kinesin motor 3A
Protein Family:Kinesin-like protein
UniProt Gene Name:KIF3A  
UniProt Entry Name:KIF3A_HUMAN
Anti-KIF3A Antibody (CAB6639)Western blot analysis of extracts of various cell lines, using KIF3A antibody (CAB6639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-KIF3A Antibody (CAB6639)Immunofluorescence analysis of MCF7 cells using KIF3A antibody (CAB6639). Blue: DAPI for nuclear staining.
Anti-KIF3A Antibody (CAB6639)Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug KIF3A antibody (CAB6639). Western blot was performed from the immunoprecipitate using KIF3A antibody (CAB6639) at a dilition of 1:1000.
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