Antibody Name:	Anti-KIF22 Antibody
Antibody SKU:	CAB15285
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 456-665 of human KIF22 (NP_015556.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A431, 293T, U-87MG, A549, HeLa, Jurkat, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 456-665 of human KIF22 (NP_015556.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QGAP LLST PKRE RMVL MKTV EEKD LEIE RLKT KQKE LEAK MLAQ KAEE KENH CPTM LRPL SHRT VTGA KPLK KAVV MPLQ LIQE QAAS PNAE IHIL KNKG RKRK LESL DALE PEEK AEDC WELQ ISPE LLAH GRQK ILDL LNEG SARD LRSL QRIG PKKA QLIV GWRE LHGP FSQV EDLE RVEG ITGK QMES FLKA NILG LAAG QRCG AS
Gene ID:	3835
Uniprot:	Q14807
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton
Calculated MW:	66kDa/73kDa
Observed MW:	73kDa

Synonyms:

KIF22, A-328A3.2, KID, KNSL4, OBP, OBP-1, OBP-2, SEMDJL2

Background:

The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	KIF22: Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2). A bone disease characterized by short stature, distinctive midface retrusion, progressive knee malalignment (genu valgum and/or varum), generalized ligamentous laxity, and mild spinal deformity. Intellectual development is not impaired. Radiographic characteristics include significantly retarded epiphyseal ossification that evolves into epiphyseal dysplasia and precocious osteoarthritis, metaphyseal irregularities and vertical striations, constricted femoral neck, slender metacarpals and metatarsals, and mild thoracolumbar kyphosis or scoliosis with normal or mild platyspondyly. The most distinctive features for differential diagnosis of SEMDJL2 are the slender metacarpals and phalanges and the progressive degeneration of carpal bones; however, these 2 features are evident only in older children and young adults. The soft consistency of cartilage in the airways leads to laryngotracheomalacia with proneness to respiratory obstruction and inspiratory stridor in infancy and childhood. Belongs to the kinesin-like protein family.
UniProt Protein Details:	Protein type:Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cytoplasm; cytosol; focal adhesion; kinesin complex; kinetochore; nucleus Molecular Function:ATPase activity; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; metaphase plate congression; microtubule-based movement; mitosis; mitotic metaphase plate congression; retrograde vesicle-mediated transport, Golgi to ER; sister chromatid cohesion Disease: Spondyloepimetaphyseal Dysplasia With Joint Laxity Type 2
NCBI Summary:	The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:	Q14807
NCBI GenInfo Identifier:	19863381
NCBI Gene ID:	3835
NCBI Accession:	Q14807.5
UniProt Secondary Accession:	Q14807,O60845, O94814, Q53F58, Q9BT46, B2R5M0, B7Z265
UniProt Related Accession:	Q14807
Molecular Weight:	66,307 Da
NCBI Full Name:	Kinesin-like protein KIF22
NCBI Synonym Full Names:	kinesin family member 22
NCBI Official Symbol:	KIF22
NCBI Official Synonym Symbols:	KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
NCBI Protein Information:	kinesin-like protein KIF22
UniProt Protein Name:	Kinesin-like protein KIF22
UniProt Synonym Protein Names:	Kinesin-like DNA-binding protein; Kinesin-like protein 4
Protein Family:	Kinesin-like protein
UniProt Gene Name:	KIF22
UniProt Entry Name:	KIF22_HUMAN

	Western blot analysis of extracts of various cell lines, using KIF22 antibody (CAB15285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human colon using KIF22 antibody (CAB15285) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using KIF22 antibody (CAB15285) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using KIF22 antibody (CAB15285) at dilution of 1:100 (40x lens).