Epigenetics & Nuclear Signaling Antibodies 3

Anti-KIAA0391 Antibody (CAB3360)

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SKU:
CAB3360
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-KIAA0391 Antibody
Antibody SKU:CAB3360
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 314-583 of human KIAA0391 (NP_055487.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 314-583 of human KIAA0391 (NP_055487.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YPGE SFAH SIKT WFES VPGK QWKG QFTT VRKS GQCS GCGK TIES IQLS PEEY ECLK GKIM RDVI DGGD QYRK TTPQ ELKR FENF IKSR PPFD VVID GLNV AKMF PKVR ESQL LLNV VSQL AKRN LRLL VLGR KHML RRSS QWSR DEME EVQK QASC FFAD DISE DDPF LLYA TLHS GNHC RFIT RDLM RDHK ACLP DAKT QRLF FKWQ QGHQ LAIV NRFP GSKL TFQR ILSY DTVV QTTG DSWH IPYD EDLV ERCS CEVP TKWL CLHQ KT
Gene ID:9692
Uniprot:O15091
Cellular Location:Mitochondrion
Calculated MW:24kDa/56kDa/65kDa/67kDa
Observed MW:67kDa
Synonyms:KIAA0391, MRPP3, PRORP
Background:
UniProt Protein Function:KIAA0391: Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 14q13.2

Cellular Component: mitochondrion

Biological Process: tRNA processing

UniProt Code:O15091
NCBI GenInfo Identifier:221222466
NCBI Gene ID:9692
NCBI Accession:O15091.2
UniProt Secondary Accession:O15091,Q86SZ4, Q86YB5, Q8N5L5, B4DXD9, B4E0S8, B4E211 C4AM93, D3DS99, D3DSA1,
UniProt Related Accession:O15091
Molecular Weight:583
NCBI Full Name:Mitochondrial ribonuclease P protein 3
NCBI Synonym Full Names:KIAA0391
NCBI Official Symbol:KIAA0391  
NCBI Official Synonym Symbols:MRPP3; PRORP  
NCBI Protein Information:mitochondrial ribonuclease P protein 3; proteinaceous RNase P; mitochondrial RNase P protein 3; mitochondrial RNase P subunit 3
UniProt Protein Name:Mitochondrial ribonuclease P protein 3
Protein Family:Mitochondrial ribonuclease P protein
UniProt Gene Name:KIAA0391  
UniProt Entry Name:MRRP3_HUMAN
Anti-KIAA0391 Antibody (CAB3360)Western blot analysis of extracts of various cell lines, using KIAA0391 antibody (CAB3360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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