Metabolism Antibodies 2
Anti-KHK Antibody (CAB7440)
- SKU:
- CAB7440
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-KHK Antibody |
| Antibody SKU: | CAB7440 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human KHK (NP_000212.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-298 of human KHK (NP_000212.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEEK QILC VGLV VLDV ISLV DKYP KEDS EIRC LSQR WQRG GNAS NSCT VLSL LGAP CAFM GSMA PGHV ADFV LDDL RRYS VDLR YTVF QTTG SVPI ATVI INEA SGSR TILY YDRS LPDV SATD FEKV DLTQ FKWI HIEG RNAS EQVK MLQR IDAH NTRQ PPEQ KIRV SVEV EKPR EELF QLFG YGDV VFVS KDVA KHLG FQSA EEAL RGLY GRVR KGAV LVCA WAEE GADA LGPD GKLL HSDA FPPP RVVD TLGA GDTF NASV IFSL SQGR SVQE ALRF GCQV AGKK CGLQ GFDG IV |
| Gene ID: | 3795 |
| Uniprot: | P50053 |
| Cellular Location: | |
| Calculated MW: | 32kDa |
| Observed MW: | 33kDa |
| Synonyms: | KHK |
| Background: | This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate. |
| NCBI Summary: | This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50053 |
| NCBI GenInfo Identifier: | 816380035 |
| NCBI Gene ID: | 3795 |
| NCBI Accession: | P50053.2 |
| UniProt Secondary Accession: | P50053,Q6IBK2, Q99532, Q9BRJ3, Q9UMN1, |
| UniProt Related Accession: | P50053 |
| Molecular Weight: | 32,730 Da |
| NCBI Full Name: | Ketohexokinase |
| NCBI Synonym Full Names: | ketohexokinase |
| NCBI Official Symbol: | KHK |
| NCBI Protein Information: | ketohexokinase |
| UniProt Protein Name: | Ketohexokinase |
| UniProt Synonym Protein Names: | Hepatic fructokinase |
| Protein Family: | Ketohexokinase |
| UniProt Gene Name: | KHK |
| UniProt Entry Name: | KHK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KHK antibody (CAB7440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human kidney using KHK antibody (CAB7440) at dilution of 1:100 (40x lens). |
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