Cell Biology Antibodies 16
Anti-Kdm6b Antibody (CAB12763)
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- SKU:
- CAB12763
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Kdm6b Antibody |
| Antibody SKU: | CAB12763 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-450 of mouse Kdm6b (NP_001017426.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SH-SY5Y, K-562, mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-450 of mouse Kdm6b (NP_001017426.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PPLP GLAI SPPF QLTK PGLW NTLH GDAW GPER KGSA PPER QEQR HSMP HSYP YPAP AYSA HPPS HRLV PNTP LGPG PRPP GAES HGCL PATR PPGS DLRE SRVQ RSRM DSSV SPAA STAC VPYA PSRP PGLP GTSS SSSS SSSS NNTG LRGV EPSP GIPG ADHY QNPA LEIS PHQA RLGP SAHS SR |
| Gene ID: | 216850 |
| Uniprot: | |
| Cellular Location: | Nucleus |
| Calculated MW: | 176kDa |
| Observed MW: | 170kDa |
| Synonyms: | KDM6B, JMJD3, Kdm6b |
| Background: |
 | Western blot analysis of extracts of various cell lines, using Kdm6b antibody (CAB12763) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Western blot analysis of extracts of mouse brain, using KDM6B antibody (CAB12763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of NIH/3T3 cells using KDM6B antibody (CAB12763) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using KDM6B antibody (CAB12763) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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