Cell Biology Antibodies 11
Anti-KDM6A Antibody (CAB8159)
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- SKU:
- CAB8159
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | KDM6A Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8159 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 550-700 of mouse KDM6A (NP_033509.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, SW620, BT-474, NIH/3T3, Mouse spleen, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 550-700 of mouse KDM6A (NP_033509.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPSV SQPG VHTA CPRQ TLAN GPFS AGHV PCST SRTL GSTD TVLI GNNH VTGS GSNG NVPY LQRN APTL PHNR TNLT SSTE EPWK NQLS NSTQ GLHK GPSS HLAG PNGE RPLS STGP SQHL QAAG SGIQ NQNG HPTL PSNS VTQG AALN HLS |
| Gene ID: | 22289 |
| Uniprot: | |
| Cellular Location: | Nucleus |
| Calculated MW: | 154kDa/157kDa |
| Observed MW: | 145kDa |
| Synonyms: | KDM6A, KABUK2, UTX, bA386N14.2 |
| Background: | This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. |
 | Western blot analysis of extracts of various cell lines, using KDM6A antibody (CAB8159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using Kdm6a antibody (CAB8159) at dilution of 1:200 (40x lens). |
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