Antibody Name:	Anti-KDM5A Antibody
Antibody SKU:	CAB7238
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human KDM5A

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	Jurkat, HeLa, MCF7

Immunogen:	Recombinant protein of human KDM5A
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5927
Uniprot:	P29375
Cellular Location:	Nucleus, nucleolus
Calculated MW:	186kDa/192kDa
Observed MW:	192kDa

Synonyms:

KDM5A, RBBP-2, RBBP2, RBP2

Background:

This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.

UniProt Protein Function:	JARID1A: Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Interacts with SUZ12; the interaction is direct. Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleolus; Oxidoreductase; Transcription factor; EC 1.14.11.-; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 12p11 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex Molecular Function:protein binding; DNA binding; zinc ion binding; transcription coactivator activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; positive regulation of transcription, DNA-dependent; multicellular organismal development; circadian regulation of gene expression; chromatin modification
NCBI Summary:	This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]
UniProt Code:	P29375
NCBI GenInfo Identifier:	215274124
NCBI Gene ID:	5927
NCBI Accession:	P29375.3
UniProt Secondary Accession:	P29375,Q4LE72, Q86XZ1, A8MV76,
UniProt Related Accession:	P29375
Molecular Weight:	1690
NCBI Full Name:	Lysine-specific demethylase 5A
NCBI Synonym Full Names:	lysine (K)-specific demethylase 5A
NCBI Official Symbol:	KDM5A
NCBI Official Synonym Symbols:	RBP2; RBBP2; RBBP-2
NCBI Protein Information:	lysine-specific demethylase 5A; histone demethylase JARID1A; retinoblastoma binding protein 2; jumonji/ARID domain-containing protein 1A; Jumonji, AT rich interactive domain 1A (RBP2-like)
UniProt Protein Name:	Lysine-specific demethylase 5A
UniProt Synonym Protein Names:	Histone demethylase JARID1A; Jumonji/ARID domain-containing protein 1A; Retinoblastoma-binding protein 2; RBBP-2
Protein Family:	Lysine-specific demethylase
UniProt Gene Name:	KDM5A
UniProt Entry Name:	KDM5A_HUMAN

	Western blot analysis of extracts of various cell lines, using KDM5A antibody at 1:500 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KDM5A antibody at a dilition of 1:500.
	Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using KDM5A antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.