Name: Anti-KDM4C Antibody (CAB8485)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB8485
Availability: OutOfStock

Antibody Name:	Anti-KDM4C Antibody
Antibody SKU:	CAB8485
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-570 of human KDM4C (NP_055876.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, Mouse lung, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-570 of human KDM4C (NP_055876.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PAST PEVK AWLQ RRRK VRKA SRSF QCAR STSK RPKA DEEE EVSD EVDG AEVP NPDS VTDD LKVS EKSE AAVK LRNT EASS EEES SASR MQVE QNLS DHIK LSGN SCLS TSVT EDIK TEDD KAYA YRSV PSIS SEAD DSIP LSSG YEKP EKSD PSEL SWPK SPES CSSV AESN GVLT EGEE SDVE SHGN GLEP GEIP AVPS GERN SFKV PSIA EGEN KTSK S
Gene ID:	23081
Uniprot:	Q9H3R0
Cellular Location:	Nucleus
Calculated MW:	91kDa/94kDa/118kDa/119kDa
Observed MW:	120kDa

Synonyms:

GASC1, JHDM3C, JMJD2C, TDRD14C, KDM4C

Background:

This gene is a member of the Jumonji domain 2 (JMJD2) family. The encoded protein is a trimethylation-specific demethylase, and converts specific trimethylated histone residues to the dimethylated form. This enzymatic action regulates gene expression and chromosome segregation. Chromosomal aberrations and changes in expression of this gene may be found in tumor cells. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	JMJD2C: the histone demethylase implicated in the epigenetic reprogramming during the early embryogenesis. Preferentially expressed in undifferentiated ES cells. JMJD2C, along with JMJD1A, regulate self-renewal in embryonic stem cells. Specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.14.11.-; Oxidoreductase; Demethylase Chromosomal Location of Human Ortholog: 9p24.1 Cellular Component: nuclear chromatin; nucleoplasm Molecular Function:androgen receptor binding; dioxygenase activity; enzyme binding; histone demethylase activity; histone demethylase activity (H3-K9 specific); zinc ion binding Biological Process: histone H3-K9 demethylation; positive regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
NCBI Summary:	This gene is a member of the Jumonji domain 2 (JMJD2) family. The encoded protein is a trimethylation-specific demethylase, and converts specific trimethylated histone residues to the dimethylated form. This enzymatic action regulates gene expression and chromosome segregation. Chromosomal aberrations and changes in expression of this gene may be found in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:	Q9H3R0
NCBI GenInfo Identifier:	97536525
NCBI Gene ID:	23081
NCBI Accession:	Q9H3R0.2
UniProt Secondary Accession:	Q9H3R0,O94877, Q2M3M0, Q5JUC9, Q5VYJ2, Q5VYJ3, B4E1Y4 B7ZL46, F5H347, F5H7P0,
UniProt Related Accession:	Q9H3R0
Molecular Weight:	94,376 Da
NCBI Full Name:	Lysine-specific demethylase 4C
NCBI Synonym Full Names:	lysine demethylase 4C
NCBI Official Symbol:	KDM4C
NCBI Official Synonym Symbols:	GASC1; JHDM3C; JMJD2C; TDRD14C
NCBI Protein Information:	lysine-specific demethylase 4C
UniProt Protein Name:	Lysine-specific demethylase 4C
UniProt Synonym Protein Names:	Gene amplified in squamous cell carcinoma 1 protein; GASC-1 protein; JmjC domain-containing histone demethylation protein 3C; Jumonji domain-containing protein 2C
UniProt Gene Name:	KDM4C
UniProt Entry Name:	KDM4C_HUMAN

Anti-KDM4C Polyclonal Antibody (CAB8485)

Western blot analysis of extracts of various cell lines, using KDM4C antibody (CAB8485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

Antibodies

Anti-KDM4C Antibody (CAB17652)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)