Epigenetics & Nuclear Signaling Antibodies 2
Anti-KDM4B Antibody (CAB5824)
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- SKU:
- CAB5824
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-KDM4B Antibody |
| Antibody SKU: | CAB5824 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human KDM4B (NP_055830.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, Jurkat, 293T, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human KDM4B (NP_055830.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGSE DHGA QNPS CKIM TFRP TMEE FKDF NKYV AYIE SQGA HRAG LAKI IPPK EWKP RQTY DDID DVVI PAPI QQVV TGQS GLFT QYNI QKKA MTVG EYRR LANS EKYC TPRH QDFD DLER KYWK NLTF VSPI YGAD ISGS LYDD DVAQ WNIG SLRT ILDM VERE CGTI IEGV NTPY LYFG MWKT TFAW HTED MDLY SINY LHFG EPKS WYAI PPEH GKRL ERLA IGFF PGSS QGCD AFLR HKMT LISP IILK KYGI PFSR |
| Gene ID: | 23030 |
| Uniprot: | O94953 |
| Cellular Location: | Nucleus |
| Calculated MW: | 50kDa/121kDa |
| Observed MW: | 110kDa |
| Synonyms: | KDM4B, JMJD2B, TDRD14B |
| Background: |
| UniProt Protein Function: | JMJD2B: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Belongs to the JHDM3 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.11.-; Oxidoreductase; Demethylase Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:histone demethylase activity |
| UniProt Code: | O94953 |
| NCBI GenInfo Identifier: | 134047803 |
| NCBI Gene ID: | 23030 |
| NCBI Accession: | O94953.4 |
| UniProt Secondary Accession: | O94953,O75274, Q6P3R5, Q9P1V1, Q9UF40, B9EGN8, D6W631 |
| UniProt Related Accession: | O94953 |
| Molecular Weight: | 50,582 Da |
| NCBI Full Name: | Lysine-specific demethylase 4B |
| NCBI Synonym Full Names: | lysine demethylase 4B |
| NCBI Official Symbol: | KDM4B |
| NCBI Official Synonym Symbols: | JMJD2B; TDRD14B |
| NCBI Protein Information: | lysine-specific demethylase 4B |
| UniProt Protein Name: | Lysine-specific demethylase 4B |
| UniProt Synonym Protein Names: | JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B |
| Protein Family: | Lysine-specific demethylase |
| UniProt Gene Name: | KDM4B |
| UniProt Entry Name: | KDM4B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KDM4B antibody (CAB5824) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
 | Immunofluorescence analysis of U2OS cells using KDM4B antibody (CAB5824). Blue: DAPI for nuclear staining. |
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