Name: Anti-KDM3A Antibody (CAB11960)
Brand: Developmental Biology
SKU: CAB11960
Availability: OutOfStock

Antibody Name:	Anti-KDM3A Antibody
Antibody SKU:	CAB11960
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human KDM3A (NP_060903.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human KDM3A (NP_060903.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LNTK SEAL RTKP DVCK AGLL SKSS QIGT GDLK ILTE PKGS CTQP KTNT DQEN RLES VPQA LTGL PKEC LPTK ASSK AELE IANP PELQ KHLE HAPS PSDV S
Gene ID:	55818
Uniprot:	Q9Y4C1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	147kDa
Observed MW:	147kDa

Synonyms:	KDM3A, JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA
Background:	This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	JMJD1A: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Belongs to the JHDM2 histone demethylase family.
UniProt Protein Details:	Protein type:EC 1.14.11.-; Demethylase; Oxidoreductase; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus Molecular Function:androgen receptor binding; dioxygenase activity; iron ion binding; transcription factor activity Biological Process: negative regulation of histone H3-K9 methylation; formaldehyde biosynthetic process; establishment and/or maintenance of chromatin architecture; spermatid nuclear elongation; histone H3-K9 demethylation; hormone-mediated signaling; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Code:	Q9Y4C1
NCBI GenInfo Identifier:	308153659
NCBI Gene ID:	55818
NCBI Accession:	Q9Y4C1.4
UniProt Related Accession:	Q9Y4C1
Molecular Weight:	147kDa
NCBI Full Name:	Lysine-specific demethylase 3A
NCBI Synonym Full Names:	lysine demethylase 3A
NCBI Official Symbol:	KDM3A
NCBI Official Synonym Symbols:	TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
NCBI Protein Information:	lysine-specific demethylase 3A
UniProt Protein Name:	Lysine-specific demethylase 3A
UniProt Synonym Protein Names:	JmjC domain-containing histone demethylation protein 2A; Jumonji domain-containing protein 1A
Protein Family:	Lysine-specific demethylase
UniProt Gene Name:	KDM3A
UniProt Entry Name:	KDM3A_HUMAN

	Western blot analysis of extracts of 293T cells, using KDM3A Antibody (CAB11960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse testis using KDM3A antibody (CAB11960) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using KDM3A antibody (CAB11960) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using KDM3A Polyclonal Antibody (CAB11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using KDM3A Polyclonal Antibody (CAB11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.