Immunology Antibodies 2

Anti-KCNN4 Antibody (CAB1974)

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SKU:
CAB1974

Description

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Antibody Name:Anti-KCNN4 Antibody
Antibody SKU:CAB1974
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 288-427 of human KCNN4 (NP_002241.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 288-427 of human KCNN4 (NP_002241.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KLEF NKAE KHVH NFMM DIQY TKEM KESA ARVL QEAW MFYK HTRR KESH AARR HQRK LLAA INAF RQVR LKHR KLRE QVNS MVDI SKMH MILY DLQQ NLSS SHRA LEKQ IDTL AGKL DALT ELLS TALG PRQL PEPS QQSK
Gene ID:3783
Uniprot:O15554
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:47kDa
Observed MW:53kDa
Synonyms:KCNN4, DHS2, IK, IK1, IKCA1, KCA4, KCa3.1, SK4, hIKCa1, hKCa4, hSK4
Background:The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.
UniProt Protein Function:KCNN4: Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
UniProt Protein Details:

Protein type:Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: apical plasma membrane; basolateral plasma membrane; cell soma; lipid raft; plasma membrane; voltage-gated potassium channel complex

Molecular Function:calcium-activated potassium channel activity; calmodulin binding; Intermediate conductance calcium-activated potassium channel activity; protein binding; protein phosphatase binding; small conductance calcium-activated potassium channel activity

Biological Process: calcium ion transport; cell volume homeostasis; defense response; immune system process; phospholipid translocation; positive regulation of protein secretion; positive regulation of T cell receptor signaling pathway; potassium ion transport; saliva secretion; stabilization of membrane potential; synaptic transmission

NCBI Summary:The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008]
UniProt Code:O15554
NCBI GenInfo Identifier:17366160
NCBI Gene ID:3783
NCBI Accession:O15554.1
UniProt Secondary Accession:O15554,Q53XR4,
UniProt Related Accession:O15554
Molecular Weight:47,696 Da
NCBI Full Name:Intermediate conductance calcium-activated potassium channel protein 4
NCBI Synonym Full Names:potassium calcium-activated channel subfamily N member 4
NCBI Official Symbol:KCNN4  
NCBI Official Synonym Symbols:IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1  
NCBI Protein Information:intermediate conductance calcium-activated potassium channel protein 4
UniProt Protein Name:Intermediate conductance calcium-activated potassium channel protein 4
UniProt Synonym Protein Names:IKCa1; IK1; KCa3.1; KCa4; Putative Gardos channel
Protein Family:Intermediate conductance calcium-activated potassium channel protein
UniProt Gene Name:KCNN4  
UniProt Entry Name:KCNN4_HUMAN
Anti-KCNN4 Antibody (CAB1974)Western blot analysis of extracts of HepG2 cells, using KCNN4 antibody (CAB1974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
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