Signal Transduction Antibodies 1

Anti-KCNMB1 Antibody (CAB10224)

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SKU:
CAB10224
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-KCNMB1 Antibody
Antibody SKU:CAB10224
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-102 of human KCNMB1 (NP_004128.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, THP-1, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-102 of human KCNMB1 (NP_004128.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TYYI LVTT VLPL YQKS VWTQ ESKC HLIE TNIR DQEE LKGK KVPQ YPCL WVNV SAAG RWAV LYHT EDTR DQNQ Q
Gene ID:3779
Uniprot:Q16558
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:14kDa/21kDa
Observed MW:18kDa
Synonyms:KCNMB1, BKbeta1, K(VCA)beta, SLO-BETA, hbeta1, hslo-beta, k(VCA)beta-1, slo-beta-1
Background:MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the product of this gene, the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits.
UniProt Protein Function:KCNMB1: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca(2+)/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate. Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: plasma membrane; voltage-gated potassium channel complex

Biological Process: detection of calcium ion; potassium ion transport; synaptic transmission

Disease: Hypertension, Diastolic, Resistance To

NCBI Summary:MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the product of this gene, the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. [provided by RefSeq, Jul 2008]
UniProt Code:Q16558
NCBI GenInfo Identifier:292495100
NCBI Gene ID:3779
NCBI Accession:Q16558.5
UniProt Secondary Accession:Q16558,O00707, O00708, P78475, Q53YR0, Q8TAX3, Q93005
UniProt Related Accession:Q16558,AAB39373
Molecular Weight:14,858 Da
NCBI Full Name:Calcium-activated potassium channel subunit beta-1
NCBI Synonym Full Names:potassium calcium-activated channel subfamily M regulatory beta subunit 1
NCBI Official Symbol:KCNMB1  
NCBI Official Synonym Symbols:hbeta1; BKbeta1; SLO-BETA; hslo-beta; K(VCA)beta; slo-beta-1; k(VCA)beta-1  
NCBI Protein Information:calcium-activated potassium channel subunit beta-1
UniProt Protein Name:Calcium-activated potassium channel subunit beta-1
UniProt Synonym Protein Names:BK channel subunit beta-1; BKbeta; BKbeta1; Hbeta1; Calcium-activated potassium channel, subfamily M subunit beta-1; Calcium-activated potassium channel subunit beta; Charybdotoxin receptor subunit beta-1; K(VCA)beta-1; Maxi K channel subunit beta-1; Slo-beta-1; Slo-beta
Protein Family:Calcium-activated potassium channel
UniProt Gene Name:KCNMB1  
UniProt Entry Name:KCMB1_HUMAN
Anti-KCNMB1 Antibody (CAB10224)Western blot analysis of extracts of various cell lines, using KCNMB1 antibody (CAB10224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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