Name: Anti-KCNK3 Antibody (CAB14745)
Brand: Signal Transduction Antibodies 1
SKU: CAB14745
Availability: OutOfStock

Antibody Name:	Anti-KCNK3 Antibody
Antibody SKU:	CAB14745
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 250-394 of human KCNK3 (NP_002237.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 250-394 of human KCNK3 (NP_002237.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NAED EKRD AEHR ALLT RNGQ AGGG GGGG SAHT TDTA SSTA AAGG GGFR NVYA EVLH FQSM CSCL WYKS REKL QYSI PMII PRDL STSD TCVE QSHS SPGG GGRY SDTP SRRC LCSG APRS AISS VSTG LHSL STFR GLMK RRSS V
Gene ID:	3777
Uniprot:	O14649
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	43kDa
Observed MW:	38kDa

Synonyms:

KCNK3, K2p3.1, OAT1, PPH4, TASK, TASK-1, TBAK1

Background:

This gene encodes a member of the superfamily of potassium channel proteins that contain two pore-forming P domains. The encoded protein is an outwardly rectifying channel that is sensitive to changes in extracellular pH and is inhibited by extracellular acidification. Also referred to as an acid-sensitive potassium channel, it is activated by the anesthetics halothane and isoflurane. Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript variants for this gene.

UniProt Protein Function:	KCNK3: pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward. Widespread expression in adult. Strongest expression in pancreas and placenta. Lower expression in brain, lung, prostate, heart, kidney, uterus, small intestine and colon. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium Chromosomal Location of Human Ortholog: 2p23 Cellular Component: cytosol; integral to plasma membrane; plasma membrane Molecular Function:ion channel activity; open rectifier potassium channel activity; potassium channel activity; potassium ion leak channel activity; protein C-terminus binding; protein heterodimerization activity; protein homodimerization activity Biological Process: brain development; potassium ion transport; reduction of cytosolic calcium ion concentration; response to drug; stabilization of membrane potential; synaptic transmission Disease: Pulmonary Hypertension, Primary, 4
NCBI Summary:	This gene encodes a member of the superfamily of potassium channel proteins that contain two pore-forming P domains. The encoded protein is an outwardly rectifying channel that is sensitive to changes in extracellular pH and is inhibited by extracellular acidification. Also referred to as an acid-sensitive potassium channel, it is activated by the anesthetics halothane and isoflurane. Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript variants for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	O14649
NCBI GenInfo Identifier:	13124040
NCBI Gene ID:	3777
NCBI Accession:	O14649.1
UniProt Secondary Accession:	O14649,Q53SU2,
UniProt Related Accession:	O14649
Molecular Weight:	43,518 Da
NCBI Full Name:	Potassium channel subfamily K member 3
NCBI Synonym Full Names:	potassium two pore domain channel subfamily K member 3
NCBI Official Symbol:	KCNK3
NCBI Official Synonym Symbols:	OAT1; PPH4; TASK; TBAK1; K2p3.1; TASK-1
NCBI Protein Information:	potassium channel subfamily K member 3
UniProt Protein Name:	Potassium channel subfamily K member 3
UniProt Synonym Protein Names:	Acid-sensitive potassium channel protein TASK-1; TWIK-related acid-sensitive K(+) channel 1; Two pore potassium channel KT3.1; Two pore K(+) channel KT3.1
Protein Family:	Potassium channel subfamily
UniProt Gene Name:	KCNK3
UniProt Entry Name:	KCNK3_HUMAN

Western blot analysis of extracts of various cell lines, using KCNK3 antibody (CAB14745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

KCNK3 Antibody (PACO07214)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)