Name: Anti-KCNJ12 Antibody (CAB14375)
Brand: Signal Transduction Antibodies 1
SKU: CAB14375
Availability: OutOfStock

Antibody Name:	Anti-KCNJ12 Antibody
Antibody SKU:	CAB14375
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 354-433 of human KCNJ12 (NP_066292.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 354-433 of human KCNJ12 (NP_066292.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TPRC SAKD LVEN KFLL PSAN SFCY ENEL AFLS RDEE DEAD GDQD GRSR DGLS PQAR HDFD RLQA GGGV LEQR PYRR ESEI
Gene ID:	3768
Uniprot:	Q14500
Cellular Location:	Cell membrane, Membrane, Multi-pass membrane protein
Calculated MW:	49kDa
Observed MW:	49kDa

Synonyms:	KCNJ12, IRK-2, IRK2, KCNJN1, Kir2.2, Kir2.2v, hIRK, hIRK1, hkir2.2x, kcnj12x
Background:	This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17.

UniProt Protein Function:	KCNJ12: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ12 subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: integral to plasma membrane; plasma membrane; intrinsic to membrane Molecular Function:inward rectifier potassium channel activity Biological Process: synaptic transmission; muscle contraction; potassium ion import; regulation of heart contraction; potassium ion transport; protein homotetramerization
NCBI Summary:	This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14500
NCBI GenInfo Identifier:	77416868
NCBI Gene ID:	3768
NCBI Accession:	Q14500.2
UniProt Secondary Accession:	Q14500,O43401, Q15756, Q8NG63,
UniProt Related Accession:	Q14500
Molecular Weight:	49,001 Da
NCBI Full Name:	ATP-sensitive inward rectifier potassium channel 12
NCBI Synonym Full Names:	potassium inwardly-rectifying channel, subfamily J, member 12
NCBI Official Symbol:	KCNJ12
NCBI Official Synonym Symbols:	IRK2; hIRK; IRK-2; hIRK1; KCNJN1; Kir2.2; Kir2.2v; kcnj12x; hkir2.2x
NCBI Protein Information:	ATP-sensitive inward rectifier potassium channel 12; inward rectifier K(+) channel Kir2.2; inward rectifier K(+) channel Kir2.6; inward rectifier K(+) channel Kir2.2v; potassium channel, inwardly rectifying subfamily J member 12; potassium inwardly-rectifying channel, subfamily J, inhibitor 1
UniProt Protein Name:	ATP-sensitive inward rectifier potassium channel 12
UniProt Synonym Protein Names:	Inward rectifier K(+) channel Kir2.2; IRK-2; Inward rectifier K(+) channel Kir2.2v; Potassium channel, inwardly rectifying subfamily J member 12
Protein Family:	ATP-sensitive inward rectifier potassium channel
UniProt Gene Name:	KCNJ12
UniProt Entry Name:	KCJ12_HUMAN

Western blot analysis of extracts of various cell lines, using KCNJ12 Rabbit pAb (CAB14375) at 1:300 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

KCNJ12 Antibody (PACO10059)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)