Antibody Name:	Anti-KARS Antibody
Antibody SKU:	CAB13772
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 298-597 of human KARS (NP_005539.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Jurkat, HeLa, 293T, Mouse heart, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 298-597 of human KARS (NP_005539.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IAPE LYHK MLVV GGID RVYE IGRQ FRNE GIDL THNP EFTT CEFY MAYA DYHD LMEI TEKM VSGM VKHI TGSY KVTY HPDG PEGQ AYDV DFTP PFRR INMV EELE KALG MKLP ETNL FETE ETRK ILDD ICVA KAVE CPPP RTTA RLLD KLVG EFLE VTCI NPTF ICDH PQIM SPLA KWHR SKEG LTER FELF VMKK EICN AYTE LNDP MRQR QLFE EQAK AKAA GDDE AMFI DENF CTAL EYGL PPTA GWGM GIDR VAMF LTDS NNIK EVLL FPAM KPED KKEN VATT DTLE STTV GTSV
Gene ID:	3735
Uniprot:	Q15046
Cellular Location:	Cell membrane, Cytoplasm, Mitochondrion, Nucleus, Peripheral membrane protein, Secreted
Calculated MW:	68kDa/71kDa
Observed MW:	75kDa

Synonyms:

KARS, CMTRIB, DFNB89, KARS1, KARS2, KRS

Background:

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; EC 6.1.1.6; Translation; Ligase Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: cytoplasm; cytosol; microtubule cytoskeleton; mitochondrial matrix; mitochondrion Molecular Function:lysine-tRNA ligase activity; protein binding Biological Process: lysyl-tRNA aminoacylation; tRNA aminoacylation for protein translation Disease: Charcot-marie-tooth Disease, Recessive Intermediate B; Deafness, Autosomal Recessive 89
NCBI Summary:	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15046
NCBI GenInfo Identifier:	20178333
NCBI Gene ID:	3735
NCBI Accession:	Q15046.3
UniProt Secondary Accession:	Q15046,O14946, Q96J25, Q9HB23, A8MSK1, D3DUK4,
UniProt Related Accession:	Q15046
Molecular Weight:	71,497 Da
NCBI Full Name:	Lysine--tRNA ligase
NCBI Synonym Full Names:	lysyl-tRNA synthetase
NCBI Official Symbol:	KARS
NCBI Official Synonym Symbols:	KRS; KARS1; KARS2; CMTRIB; DFNB89
NCBI Protein Information:	lysine--tRNA ligase
UniProt Protein Name:	Lysine--tRNA ligase
UniProt Synonym Protein Names:	Lysyl-tRNA synthetase; LysRS
UniProt Gene Name:	KARS
UniProt Entry Name:	SYK_HUMAN

	Western blot analysis of extracts of various cell lines, using KARS antibody (CAB13772) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of U-2 OS cells using KARS Rabbit pAb (CAB13772) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.