Name: Anti-JARID1B Antibody (CAB14104)
Brand: Cell Biology Antibodies 4
SKU: CAB14104
Availability: OutOfStock

Antibody Name:	Anti-JARID1B Antibody
Antibody SKU:	CAB14104
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 784-883 of human JARID1B (NP_006609.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 784-883 of human JARID1B (NP_006609.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EESE MKKF PDND LLRH LRLV TQDA EKCA SVAQ QLLN GKRQ TRYR SGGG KSQN QLTV NELR QFVT QLYA LPCV LSQT PLLK DLLN RVED FQQH SQKL LSEE
Gene ID:	10765
Uniprot:	Q9UGL1
Cellular Location:	Nucleus
Calculated MW:	175kDa/179kDa
Observed MW:	176kDa

Synonyms:

KDM5B, CT31, JARID1B, PLU-1, PLU1, PPP1R98, PUT1, RBBP2H1A, RBP2-H1

Background:

This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	JARID1B: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Demethylase; Cancer Testis Antigen (CTA); EC 1.14.11.-; Oxidoreductase; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:histone demethylase activity; histone demethylase activity (H3-K4 specific); protein binding; transcription corepressor activity; transcription factor activity
NCBI Summary:	This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing resultsi n multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:	Q9UGL1
NCBI GenInfo Identifier:	296439317
NCBI Gene ID:	10765
NCBI Accession:	Q9UGL1.3
UniProt Secondary Accession:	Q9UGL1,O95811, Q15752, Q9Y3Q5,
UniProt Related Accession:	Q9UGL1
Molecular Weight:	179,409 Da
NCBI Full Name:	Lysine-specific demethylase 5B
NCBI Synonym Full Names:	lysine demethylase 5B
NCBI Official Symbol:	KDM5B
NCBI Official Synonym Symbols:	CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBP2-H1; RBBP2H1A
NCBI Protein Information:	lysine-specific demethylase 5B
UniProt Protein Name:	Lysine-specific demethylase 5B
UniProt Synonym Protein Names:	Cancer/testis antigen 31; CT31; Histone demethylase JARID1B; Jumonji/ARID domain-containing protein 1B; PLU-1; Retinoblastoma-binding protein 2 homolog 1; RBP2-H1
Protein Family:	Lysine-specific demethylase
UniProt Gene Name:	KDM5B
UniProt Entry Name:	KDM5B_HUMAN

Western blot analysis of extracts of various cell lines, using KDM5B antibody (CAB14104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-KDM5B Antibody (CAB7772)[KO Validated]

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)