Cardiovascular Antibodies

Anti-JAM3 Antibody (CAB14942)

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SKU:
CAB14942
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-JAM3 Antibody
Antibody SKU:CAB14942
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JAM3 (NP_116190.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T, HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JAM3 (NP_116190.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MALR RPPR LRLC ARLP DFFL LLLF RGCL IGAV NLKS SNRT PVVQ EFES VELS CIIT DSQT SDPR IEWK KIQD EQTT YVFF DNKI QGDL AGRA EILG KTSL
Gene ID:83700
Uniprot:Q9BX67
Cellular Location:Cell junction, Cell membrane, Secreted, Single-pass type I membrane protein, desmosome, extracellular space
Calculated MW:29kDa/35kDa
Observed MW:37kDa
Synonyms:JAM3, JAM-2, JAM-3, JAM-C, JAMC
Background:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:JAM3: Participates in cell-cell adhesion. It is a counter- receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC). A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: desmosome; extracellular space; Golgi apparatus; plasma membrane

Molecular Function:integrin binding; protein binding

Biological Process: angiogenesis; extracellular matrix organization and biogenesis; leukocyte migration

Disease: Hemorrhagic Destruction Of The Brain, Subependymal Calcification, And Cataracts

NCBI Summary:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
UniProt Code:Q9BX67
NCBI GenInfo Identifier:51701611
NCBI Gene ID:83700
NCBI Accession:Q9BX67.1
UniProt Secondary Accession:Q9BX67,Q8WWL8, Q96FL1, B3KWG9,
UniProt Related Accession:Q9BX67
Molecular Weight:29,223 Da
NCBI Full Name:Junctional adhesion molecule C
NCBI Synonym Full Names:junctional adhesion molecule 3
NCBI Official Symbol:JAM3  
NCBI Official Synonym Symbols:JAMC; JAM-2; JAM-3; JAM-C  
NCBI Protein Information:junctional adhesion molecule C
UniProt Protein Name:Junctional adhesion molecule C
UniProt Synonym Protein Names:JAM-2; Junctional adhesion molecule 3; JAM-3
Protein Family:Junctional adhesion molecule
UniProt Gene Name:JAM3  
UniProt Entry Name:JAM3_HUMAN
Anti-JAM3 Antibody (CAB14942)Western blot analysis of extracts of various cell lines, using JAM3 antibody (CAB14942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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