Cell Biology Antibodies 1

Anti-JAK1 Antibody (CAB0715)

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SKU:
CAB0715
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-JAK1 Antibody
Antibody SKU:CAB0715
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide of human JAK1
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:HeLa, Jurkat, U-87MG, LO2
Immunogen:A synthetic peptide of human JAK1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3716
Uniprot:P23458
Cellular Location:Endomembrane system, Peripheral membrane protein
Calculated MW:133kDa
Observed MW:115kDa
Synonyms:JAK1, JAK1A, JAK1B, JTK3
Background:This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:JAK1: a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways. Couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. A selective Jak1 inhibitor induces apoptosis in NRP-154 prostate cancer cell line. A single mutation seen in tyrosine kinome screen of colon cancers. Activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. Inhibitor: Piceatannol.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.10.2; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; JakA family

Chromosomal Location of Human Ortholog: 1p32.3-p31.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; endomembrane system; extrinsic to internal side of plasma membrane; focal adhesion; nucleus

Molecular Function:ATP binding; CCR5 chemokine receptor binding; growth hormone receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding; ubiquitin protein ligase binding

Biological Process: cell differentiation; cell migration; innate immune response; MAPKKK cascade; positive regulation of GTPase activity; protein amino acid phosphorylation; regulation of cell proliferation; response to antibiotic; transmembrane receptor protein tyrosine kinase signaling pathway

NCBI Summary:This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:P23458
NCBI GenInfo Identifier:215274013
NCBI Gene ID:3716
NCBI Accession:P23458.2
UniProt Secondary Accession:P23458,Q59GQ2, Q9UD26,
UniProt Related Accession:P23458
Molecular Weight:
NCBI Full Name:Tyrosine-protein kinase JAK1
NCBI Synonym Full Names:Janus kinase 1
NCBI Official Symbol:JAK1  
NCBI Official Synonym Symbols:JTK3; JAK1A; JAK1B  
NCBI Protein Information:tyrosine-protein kinase JAK1
UniProt Protein Name:Tyrosine-protein kinase JAK1
UniProt Synonym Protein Names:Janus kinase 1; JAK-1
Protein Family:Tyrosine-protein kinase
UniProt Gene Name:JAK1  
UniProt Entry Name:JAK1_HUMAN
Anti-JAK1 Antibody (CAB0715)Western blot analysis of extracts of various cell lines, using JAK1 antibody (CAB0715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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