Name: Anti-JAG1 Antibody (CAB12733)
Brand: Signal Transduction Antibodies 1
SKU: CAB12733
Availability: OutOfStock

Antibody Name:	Anti-JAG1 Antibody
Antibody SKU:	CAB12733
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 34-180 of human JAG1 (NP_000205.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 34-180 of human JAG1 (NP_000205.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QFEL EILS MQNV NGEL QNGN CCGG ARNP GDRK CTRD ECDT YFKV CLKE YQSR VTAG GPCS FGSG STPV IGGN TFNL KASR GNDR NRIV LPFS FAWP RSYT LLVE AWDS SNDT VQPD SIIE KASH SGMI NPSR QWQT LKQN TGVA HFE
Gene ID:	182
Uniprot:	P78504
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	116kDa/133kDa
Observed MW:	160kDa

Synonyms:	JAG1, AGS, AGS1, AHD, AWS, CD339, HJ1, JAGL1, jagged 1
Background:	The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

UniProt Protein Function:	JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 20p12.2 Cellular Component: adherens junction; apical plasma membrane; membrane; plasma membrane Molecular Function:Notch binding; phospholipid binding; protein binding Biological Process: Notch receptor processing; Notch signaling pathway; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; T cell mediated immunity Disease: Alagille Syndrome 1; Tetralogy Of Fallot
NCBI Summary:	The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]
UniProt Code:	P78504
NCBI GenInfo Identifier:	20455033
NCBI Gene ID:	182
NCBI Accession:	P78504.3
UniProt Secondary Accession:	P78504,O14902, O15122, Q15816, A0AV43, B4DYR1, E9PCF9
UniProt Related Accession:	P78504
Molecular Weight:	134kDa
NCBI Full Name:	Protein jagged-1
NCBI Synonym Full Names:	jagged 1
NCBI Official Symbol:	JAG1
NCBI Official Synonym Symbols:	AGS; AHD; AWS; HJ1; AGS1; CD339; JAGL1
NCBI Protein Information:	protein jagged-1
UniProt Protein Name:	Protein jagged-1
UniProt Synonym Protein Names:	CD_antigen: CD339
Protein Family:	Protein jagged
UniProt Gene Name:	JAG1

	Western blot analysis of extracts of HeLa cells, using JAG1 antibody (CAB12733) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of NIH/3T3 cells using JAG1 antibody (CAB12733) at dilution of 1:100. Blue: DAPI for nuclear staining.