Cell Biology Antibodies 12

Anti-IYD Antibody (CAB9206)

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SKU:
CAB9206
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-IYD Antibody
Antibody SKU:CAB9206
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-214 of human IYD (NP_981932.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse lung, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-214 of human IYD (NP_981932.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DRSM EKKK GEPR TRAE ARPW VDED LKDS SDLH QAEE DADE WQES EENV EHIP FSHN HYPE KEMV KRSQ EFYE LLNK RRSV RFIS NEQV PMEV IDNV IRTA GTAP SGAH TEPW TFVV VKDP DVKH KIRK IIEE EEEI NYMK RMGH RWVT DLKK LRTN WIKE YLDT APIL ILIF KQVH GFAA NGKK KVHY YNE
Gene ID:389434
Uniprot:Q6PHW0
Cellular Location:Cell membrane, Single-pass membrane protein
Calculated MW:22kDa/28kDa/31kDa/32kDa/33kDa
Observed MW:25kDa
Synonyms:IYD, C6orf71, DEHAL1, IYD-1, TDH4
Background:This gene encodes an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:IYD: Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine. Defects in IYD are the cause of thyroid dyshormonogenesis 4 (TDH4). A disorder due to thyroid dyshormonogenesis, causing severe hypothyroidism, goiter, excessive levels of iodotyrosine in serum and urine, and variable mental deficits derived from unrecognized and untreated hypothyroidism. Belongs to the nitroreductase family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; EC 1.22.1.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function:iodide peroxidase activity; oxidoreductase activity

Biological Process: thyroid hormone generation

Disease: Thyroid Dyshormonogenesis 4

NCBI Summary:This gene encodes an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Code:Q6PHW0
NCBI GenInfo Identifier:33989988
NCBI Gene ID:389434
NCBI Accession:BC056253
UniProt Secondary Accession:Q6PHW0,Q2VPW0, Q2VPW1, Q5F1L5, Q5F1L6, Q5THM4, Q6ZP69 Q7Z7D7, Q7Z7D8, C9JFW2,
UniProt Related Accession:Q6PHW0
Molecular Weight:22,460 Da
NCBI Full Name:Homo sapiens iodotyrosine deiodinase, mRNA
NCBI Synonym Full Names:iodotyrosine deiodinase
NCBI Official Symbol:IYD  
NCBI Official Synonym Symbols:TDH4; DEHAL1; C6orf71; dJ422F24.1  
NCBI Protein Information:iodotyrosine dehalogenase 1; IYD-1
UniProt Protein Name:Iodotyrosine dehalogenase 1
Protein Family:Iodotyrosine deiodinase
UniProt Gene Name:IYD  
UniProt Entry Name:IYD1_HUMAN
Anti-IYD Polyclonal Antibody (CAB9206)Western blot analysis of extracts of various cell lines, using IYD antibody (CAB9206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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