Cell Biology Antibodies 11

Anti-IVL Antibody (CAB8026)

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SKU:
CAB8026
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:IVL Rabbit Polyclonal Antibody
Antibody SKU:CAB8026
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 416-585 of human IVL (NP_005538.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A375, Jurkat, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 416-585 of human IVL (NP_005538.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QERQ VEHL EQQV GQLK HLEE QEGQ LKHL EQQQ GQLE VPEQ QVGQ PKNL EQEE KQLE LPEQ QEGQ VKHL EKQE AQLE LPEQ QVGQ PKHL EQQE KHLE HPEQ QDGQ LKHL EQQE GQLK DLEQ QKGQ LEQP VFAP APGQ VQDI QPAL PTKG EVLL PVEH QQQK QEVQ WPPK HK
Gene ID:3713
Uniprot:P07476
Cellular Location:Cytoplasm
Calculated MW:68kDa
Observed MW:80kDa
Synonyms:IVL
Background:Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
UniProt Protein Function:IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cornified envelope

Molecular Function:protein binding, bridging; structural molecule activity

Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B

NCBI Summary:Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
UniProt Code:P07476
NCBI GenInfo Identifier:254763301
NCBI Gene ID:3713
NCBI Accession:P07476.2
UniProt Secondary Accession:P07476,Q5T7P4,
UniProt Related Accession:P07476
Molecular Weight:68,479 Da
NCBI Full Name:Involucrin
NCBI Synonym Full Names:involucrin
NCBI Official Symbol:IVL  
NCBI Protein Information:involucrin
UniProt Protein Name:Involucrin
Protein Family:Involucrin
UniProt Gene Name:IVL  
UniProt Entry Name:INVO_HUMAN
Anti-IVL Polyclonal Antibody (CAB8026)Western blot analysis of extracts of various cell lines, using IVL antibody (CAB8026) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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