Cell Biology Antibodies 1

Anti-ITGB4 Antibody (CAB0857)

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SKU:
CAB0857
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ITGB4 Antibody
Antibody SKU:CAB0857
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-310 of human ITGB4 (NP_000204.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A431, BxPC-3, mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-310 of human ITGB4 (NP_000204.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NRCK KAPV KSCT ECVR VDKD CAYC TDEM FRDR RCNT QAEL LAAG CQRE SIVV MESS FQIT EETQ IDTT LRRS QMSP QGLR VRLR PGEE RHFE LEVF EPLE SPVD LYIL MDFS NSMS DDLD NLKK MGQN LARV LSQL TSDY TIGF GKFV DKVS VPQT DMRP EKLK EPWP NSDP PFSF KNVI SLTE DVDE FRNK LQGE RISG NLDA PEGG FDAI LQTA VCTR DIGW RPDS THLL VFST ESAF HYEA DGAN VLAG IMSR NDER CHLD TTGT YTQY RTQD YPSV PTL
Gene ID:3691
Uniprot:P16144
Cellular Location:Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein, hemidesmosome
Calculated MW:106kDa/194kDa/195kDa/200kDa/202kDa
Observed MW:202kDa
Synonyms:ITGB4, CD104, GP150
Background:Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:ITGB4: integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Five alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cell surface; hemidesmosome; leading edge; plasma membrane; basal plasma membrane; basement membrane; integrin complex; receptor complex

Molecular Function:protein binding; G-protein-coupled receptor binding; receptor activity

Biological Process: skin development; integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; hemidesmosome assembly; filopodium formation; gut development; cell-matrix adhesion; cell motility involved in cell locomotion; response to wounding; mesodermal cell differentiation; autophagy; cell adhesion

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Junctionalis With Pyloric Atresia

NCBI Summary:Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P16144
NCBI GenInfo Identifier:317373584
NCBI Gene ID:3691
NCBI Accession:P16144.5
UniProt Secondary Accession:P16144,O14690, O14691, O15339, O15340, O15341, Q0VF97 Q9UIQ4, A0AVL6,
UniProt Related Accession:P16144
Molecular Weight:106,779 Da
NCBI Full Name:Integrin beta-4
NCBI Synonym Full Names:integrin, beta 4
NCBI Official Symbol:ITGB4  
NCBI Official Synonym Symbols:CD104  
NCBI Protein Information:integrin beta-4; GP150; CD104 antigen; integrin beta-4 subunit
UniProt Protein Name:Integrin beta-4
UniProt Synonym Protein Names:GP150; CD_antigen: CD104
Protein Family:Integrin
UniProt Gene Name:ITGB4  
UniProt Entry Name:ITB4_HUMAN
Anti-ITGB4 Antibody (CAB0857)Western blot analysis of extracts of various cell lines, using ITGB4 antibody (CAB0857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ITGB4 Antibody (CAB0857)Western blot analysis of extracts of various cells, using ITGB4 antibody (CAB0857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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