Cell Biology Antibodies 8

Anti-ITGB1 Antibody (CAB2217)

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SKU:
CAB2217
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ITGB1 Antibody
Antibody SKU:CAB2217
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ITGB1 (NP_391988.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, Jurkat, Mouse skeletal muscle, Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ITGB1 (NP_391988.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EASN GQIC NGRG ICEC GVCK CTDP KFQG QTCE MCQT CLGV CAEH KECV QCRA FNKG EKKD TCTQ ECSY FNIT KVES RDKL PQPV QPDP VSHC KEKD VDDC W
Gene ID:3688
Uniprot:P05556
Cellular Location:Cell junction, Cell membrane, Cell projection, Cleavage furrow, Melanosome, Recycling endosome, Single-pass type I membrane protein, invadopodium membrane, lamellipodium, ruffle membrane, ruffle, sarcolemma
Calculated MW:87kDa/88kDa/91kDa
Observed MW:100-135kDa
Synonyms:CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, Integrin beta 1, ITGB1, Integrin Beta1
Background:Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
UniProt Protein Function:ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin.. Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).
UniProt Protein Details:

Protein type:Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 10p11.22

Cellular Component: cell surface; cell-cell adherens junction; cytoplasm; filopodium; focal adhesion; lipid raft; membrane; neuromuscular junction; plasma membrane; receptor complex; ruffle; sarcolemma

Molecular Function:actin binding; C-X3-C chemokine binding; cell adhesion molecule binding; coreceptor activity; fibronectin binding; protease binding; protein binding; protein complex binding; protein heterodimerization activity

Biological Process: B cell differentiation; calcium-independent cell-matrix adhesion; cell adhesion mediated by integrin; cell migration; cell-cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; cellular defense response; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; homophilic cell adhesion; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; mesodermal cell differentiation; positive regulation of apoptosis; positive regulation of GTPase activity; receptor internalization; regulation of immune response; stress fiber formation; transforming growth factor beta receptor signaling pathway

NCBI Summary:Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P05556
NCBI GenInfo Identifier:218563324
NCBI Gene ID:3688
NCBI Accession:P05556.2
UniProt Secondary Accession:P05556,P78466, P78467, Q13089, Q13090, Q13091, Q13212 A8K6N2, D3DRX9, D3DRY3, D3DRY4, D3DRY5,
UniProt Related Accession:P05556
Molecular Weight:88,884 Da
NCBI Full Name:Integrin beta-1
NCBI Synonym Full Names:integrin subunit beta 1
NCBI Official Symbol:ITGB1  
NCBI Official Synonym Symbols:CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA  
NCBI Protein Information:integrin beta-1
UniProt Protein Name:Integrin beta-1
UniProt Synonym Protein Names:Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD_antigen: CD29
UniProt Gene Name:ITGB1  
Anti-ITGB1 Antibody (CAB2217)Western blot analysis of extracts of various cell lines, using ITGB1 antibody (CAB2217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-ITGB1 Antibody (CAB2217)Immunofluorescence analysis of NIH-3T3 cells using ITGB1 Rabbit pAb (CAB2217) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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