Antibody Name:	Anti-ITGAX Antibody
Antibody SKU:	CAB1508
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human ITGAX (NP_000878.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human ITGAX (NP_000878.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TSPS QLLA CGPT VHHE CGRN MYLT GLCF LLGP TQLT QRLP VSRQ ECPR QEQD IVFL IDGS GSIS SRNF ATMM NFVR AVIS QFQR PSTQ FSLM QFSN KFQT HFTF EEFR RSSN PLSL LASV HQLQ GFTY TATA IQNV VHRL FHAS YGAR RDAA KILI VITD GKKE GDSL DYKD VIPM ADAA GIIR YAIG VGLA FQNR NSWK ELND IASK PSQE HIFK VEDF DALK DIQN QLKE KIFA IEGT ETTS SSSF ELE
Gene ID:	3687
Uniprot:	P20702
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	127kDa
Observed MW:	145KDa

Synonyms:

ITGAX, CD11C, SLEB6

Background:

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cell surface; membrane; plasma membrane Molecular Function:protein binding; receptor activity Biological Process: cell adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; leukocyte migration; organ morphogenesis Disease: Systemic Lupus Erythematosus
NCBI Summary:	This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Code:	P20702
NCBI GenInfo Identifier:	146345441
NCBI Gene ID:	3687
NCBI Accession:	P20702.3
UniProt Secondary Accession:	P20702,Q8IVA6,
UniProt Related Accession:	P20702
Molecular Weight:	127,829 Da
NCBI Full Name:	Integrin alpha-X
NCBI Synonym Full Names:	integrin subunit alpha X
NCBI Official Symbol:	ITGAX
NCBI Official Synonym Symbols:	CD11C; SLEB6
NCBI Protein Information:	integrin alpha-X
UniProt Protein Name:	Integrin alpha-X
UniProt Synonym Protein Names:	CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD_antigen: CD11c
UniProt Gene Name:	ITGAX

	Western blot analysis of extracts of THP-1 cells, using ITGAX Rabbit pAb (CAB1508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat lung using ITGAX antibody (CAB1508) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using ITGAX antibody (CAB1508) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using ITGAX antibody (CAB1508) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using ITGAX antibody (CAB1508) at dilution of 1:100 (40x lens).