Cell Biology Antibodies 10
Anti-ITGA5 Antibody (CAB6209)
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- SKU:
- CAB6209
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ITGA5 Antibody |
| Antibody SKU: | CAB6209 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 750-1000 of human ITGA5 (NP_002196.4). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, NIH/3T3, HT-1080, Rat heart, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 750-1000 of human ITGA5 (NP_002196.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TVPH LRDT KKTI QFDF QILS KNLN NSQS DVVS FRLS VEAQ AQVT LNGV SKPE AVLF PVSD WHPR DQPQ KEED LGPA VHHV YELI NQGP SSIS QGVL ELSC PQAL EGQQ LLYV TRVT GLNC TTNH PINP KGLE LDPE GSLH HQQK REAP SRSS ASSG PQIL KCPE AECF RLRC ELGP LHQQ ESQS LQLH FRVW AKTF LQRE HQPF SLQC EAVY KALK MPYR ILPR QLPQ KERQ VATA VQWT KAEG SYGV PLW |
| Gene ID: | 3678 |
| Uniprot: | P08648 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 114kDa |
| Observed MW: | 135kDa |
| Synonyms: | CD49e, FNRA, VLA-5, VLA5A, Integrin alpha 5, ITGA5 |
| Background: | The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. |
| UniProt Protein Function: | ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 12q11-q13 Cellular Component: cell surface; focal adhesion; intercellular junction; plasma membrane; ruffle Molecular Function:platelet-derived growth factor receptor binding; protein binding; vascular endothelial growth factor receptor 2 binding Biological Process: angiogenesis; cell adhesion; cell-substrate adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; leukocyte migration; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; wound healing, spreading of epidermal cells |
| NCBI Summary: | The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015] |
| UniProt Code: | P08648 |
| NCBI GenInfo Identifier: | 23831237 |
| NCBI Gene ID: | 3678 |
| NCBI Accession: | P08648.2 |
| UniProt Secondary Accession: | P08648,Q96HA5, |
| UniProt Related Accession: | P08648 |
| Molecular Weight: | 114,536 Da |
| NCBI Full Name: | Integrin alpha-5 |
| NCBI Synonym Full Names: | integrin subunit alpha 5 |
| NCBI Official Symbol: | ITGA5 |
| NCBI Official Synonym Symbols: | FNRA; CD49e; VLA-5; VLA5A |
| NCBI Protein Information: | integrin alpha-5 |
| UniProt Protein Name: | Integrin alpha-5 |
| UniProt Synonym Protein Names: | CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD_antigen: CD49e |
| Protein Family: | Integrin |
| UniProt Gene Name: | ITGA5 |
| UniProt Entry Name: | ITA5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ITGA5 antibody (CAB6209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using ITGA5 antibody (CAB6209) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunofluorescence analysis of Human placenta using ITGA5 antibody (CAB6209) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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