Antibody Name:	Anti-ITGA2 Antibody [KO Validated]
Antibody SKU:	CAB7629
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human ITGA2

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HT-1080

Immunogen:	Recombinant protein of human ITGA2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3673
Uniprot:	P17301
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	129kDa
Observed MW:	150kDa

Synonyms:

BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2, ITGA2

Background:

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.
UniProt Protein Details:	Protein type:Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 5q11.2 Cellular Component: cell surface; focal adhesion; integrin complex; plasma membrane Molecular Function:collagen binding; protein binding; protein complex binding Biological Process: blood coagulation; cell adhesion; cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; extracellular matrix organization and biogenesis; integrin-mediated signaling pathway; mesodermal cell differentiation; organ morphogenesis; substrate-bound cell migration Disease: Bleeding Disorder, Platelet-type, 9
NCBI Summary:	This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Code:	P17301
NCBI GenInfo Identifier:	124942
NCBI Gene ID:	3673
NCBI Accession:	P17301.1
UniProt Secondary Accession:	P17301,Q14595,
UniProt Related Accession:	P17301
Molecular Weight:	129,295 Da
NCBI Full Name:	Integrin alpha-2
NCBI Synonym Full Names:	integrin subunit alpha 2
NCBI Official Symbol:	ITGA2
NCBI Official Synonym Symbols:	BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
NCBI Protein Information:	integrin alpha-2
UniProt Protein Name:	Integrin alpha-2
UniProt Synonym Protein Names:	CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; CD_antigen: CD49b
Protein Family:	Integrin
UniProt Gene Name:	ITGA2
UniProt Entry Name:	ITA2_HUMAN

	Western blot analysis of extracts of various cell lines, using ITGA2 antibody (CAB7629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (CAB7629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (CAB7629) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (CAB7629) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using ITGA2 antibody (CAB7629) at dilution of 1:100. Blue: DAPI for nuclear staining.