KO Validated Antibodies 1

Anti-ITCH Antibody (CAB15612)[KO Validated]

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SKU:
CAB15612
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-ITCH Antibody [KO Validated]
Antibody SKU:CAB15612
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ITCH (NP_001244066.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, THP-1, NIH/3T3, C6, Mouse lung, Mouse kidney, Rat lung, Rat spleen
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ITCH (NP_001244066.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QSKK TEKC NNTN SPKW KQPL TVIV TPVS KLHF RVWS HQTL KSDV LLGT AALD IYET LKSN NMKL EEVV VTLQ LGGD KEPT ETIG DLSI CLDG LQLE SEVV T
Gene ID:83737
Uniprot:Q96J02
Cellular Location:
Calculated MW:103kDa
Observed MW:103kDa
Synonyms:ITCH, ADMFD, AIF4, AIP4, NAPP1
Background:This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:14602072, PubMed:17028573, PubMed:16387660, PubMed:18718448, PubMed:18718449, PubMed:11046148, PubMed:19592251, PubMed:19116316, PubMed:19881509, PubMed:20491914, PubMed:20392206, PubMed:20068034, PubMed:23146885, PubMed:24790097, PubMed:25631046). Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation (PubMed:17028573, PubMed:18718448, PubMed:19131965, PubMed:19881509). Involved in the control of inflammatory signaling pathways (PubMed:19131965). Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways (PubMed:19131965). Promotes the association of the complex after TNF stimulation (PubMed:19131965). Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains (PubMed:19131965). This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1 (PubMed:19131965). Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways (PubMed:19592251). Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response (PubMed:18718448, PubMed:20491914). Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages (PubMed:18718448). Mediates JUN ubiquitination and degradation. Mediates JUNB ubiquitination and degradation (PubMed:16387660). Critical regulator of type 2 helper T (Th2) cell cytokine production by inducing JUNB ubiquitination and degradation. Involved in the negative regulation of MAVS-dependent cellular antiviral responses (PubMed:19881509). Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation (PubMed:19881509). Following ligand stimulation, regulates sorting of Wnt receptor FZD4 to the degradative endocytic pathway probably by modulating PI42KA activity (PubMed:23146885). Ubiquitinates PI4K2A and negatively regulates its catalytic activity (PubMed:23146885). Ubiquitinates chemokine receptor CXCR4 and regulates sorting of CXCR4 to the degradative endocytic pathway following ligand stimulation by ubiquitinating endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed:14602072, PubMed:23146885). Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination (PubMed:17028573, PubMed:18628966, PubMed:23886940). Ubiquitinates SNX9 (PubMed:20491914). Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP (PubMed:20068034). Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID (PubMed:20392206). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).
NCBI Summary:This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:Q96J02
NCBI GenInfo Identifier:37537897
NCBI Gene ID:83737
NCBI Accession:Q96J02.2
UniProt Secondary Accession:Q96J02,O43584, Q5QP37, Q5TEL0, Q96F66, Q9BY75, Q9H451 Q9H4U5, A6NEW4, B4E234, E1P5P3, F5H217,
UniProt Related Accession:Q96J02
Molecular Weight:102 kDa
NCBI Full Name:E3 ubiquitin-protein ligase Itchy homolog
NCBI Synonym Full Names:itchy E3 ubiquitin protein ligase
NCBI Official Symbol:ITCH
NCBI Official Synonym Symbols:AIF4; AIP4; ADMFD; NAPP1
NCBI Protein Information:E3 ubiquitin-protein ligase Itchy homolog
UniProt Protein Name:E3 ubiquitin-protein ligase Itchy homolog
UniProt Synonym Protein Names:Atrophin-1-interacting protein 4; AIP4; HECT-type E3 ubiquitin transferase Itchy homolog; NFE2-associated polypeptide 1; NAPP1
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:ITCH
Anti-ITCH Antibody [KO Validated] (CAB15612)Western blot analysis of extracts of various cell lines, using ITCH antibody (CAB15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ITCH Antibody [KO Validated] (CAB15612)Western blot analysis of extracts from normal (control) and ITCH knockout (KO) 293T cells, using ITCH antibody (CAB15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-ITCH Antibody [KO Validated] (CAB15612)Immunohistochemistry of paraffin-embedded rat brain using ITCH antibody (CAB15612) at dilution of 1:100 (40x lens).
Anti-ITCH Antibody [KO Validated] (CAB15612)Immunohistochemistry of paraffin-embedded human lung cancer using ITCH antibody (CAB15612) at dilution of 1:100 (40x lens).
Anti-ITCH Antibody [KO Validated] (CAB15612)Immunohistochemistry of paraffin-embedded human placenta using ITCH antibody (CAB15612) at dilution of 1:100 (40x lens).
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