Antibody Name:	Anti-IRS2 Antibody
Antibody SKU:	CAB7945
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-650 of human IRS2 (NP_003740.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, Mouse brain, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-650 of human IRS2 (NP_003740.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LRAF CSHR SNTP ESIA ETPP ARDG GGGG EFYG YMTM DRPL SHCG RSYR RVSG DAAQ DLDR GLRK RTYS LTTP ARQR PVPQ PSSA SLDE YTLM RATF SGSA GRLC PSCP ASSP KVAY HPYP EDYG DIEI GSHR SSSS NLGA D
Gene ID:	8660
Uniprot:	Q9Y4H2
Cellular Location:	Cytoplasm, cytosol
Calculated MW:	137kDa
Observed MW:	170kDa

Synonyms:

IRS2, IRS-2

Background:

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

UniProt Protein Function:	IRS2: May mediate the control of various cellular processes by insulin. Interacts with PHIP.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 13q34 Cellular Component: cytosol; plasma membrane Molecular Function:1-phosphatidylinositol-3-kinase activity; insulin receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoinositide 3-kinase binding; protein binding; Ras guanyl-nucleotide exchange factor activity Biological Process: cellular response to insulin stimulus; glucose metabolic process; insulin receptor signaling pathway; lipid homeostasis; MAPKKK cascade; negative regulation of B cell apoptosis; negative regulation of kinase activity; phosphoinositide-mediated signaling; positive regulation of B cell proliferation; positive regulation of fatty acid beta-oxidation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of insulin secretion; regulation of lipid metabolic process; regulation of phosphoinositide 3-kinase cascade; response to glucose stimulus; signal transduction Disease: Diabetes Mellitus, Noninsulin-dependent
NCBI Summary:	This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y4H2
NCBI GenInfo Identifier:	62298062
NCBI Gene ID:	8660
NCBI Accession:	Q9Y4H2.2
UniProt Secondary Accession:	Q9Y4H2,Q96RR2, Q9BZG0, Q9Y6I5,
UniProt Related Accession:	Q9Y4H2
Molecular Weight:	137,334 Da
NCBI Full Name:	Insulin receptor substrate 2
NCBI Synonym Full Names:	insulin receptor substrate 2
NCBI Official Symbol:	IRS2
NCBI Official Synonym Symbols:	IRS-2
NCBI Protein Information:	insulin receptor substrate 2
UniProt Protein Name:	Insulin receptor substrate 2
Protein Family:	Insulin receptor substrate
UniProt Gene Name:	IRS2
UniProt Entry Name:	IRS2_HUMAN

	Western blot analysis of extracts of various cell lines, using IRS2 antibody (CAB7945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse stomach using IRS2 antibody (CAB7945) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using IRS2 antibody (CAB7945) at dilution of 1:100 (40x lens).