Immunology Antibodies 1
Anti-IRF9 Antibody (CAB1004)
- SKU:
- CAB1004
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-IRF9 Antibody |
| Antibody SKU: | CAB1004 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 113-255 of human IRF9 (NP_006075.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | OVCAR3, DU145 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 113-255 of human IRF9 (NP_006075.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QLLP PGIV SGQP GTQK VPSK RQHS SVSS ERKE EEDA MQNC TLSP SVLQ DSLN NEEE GASG GAVH SDIG SSSS SSSP EPQE VTDT TEAP FQGD QRSL EFLL PPEP DYSL LLTF IYNG RVVG EAQV QSLD CRLV AEPS GSES SME |
| Gene ID: | 10379 |
| Uniprot: | Q00978 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 43kDa |
| Observed MW: | 43kDa |
| Synonyms: | IRF9, IRF-9, ISGF3, ISGF3G, p48 |
| Background: |
| UniProt Protein Function: | IRF9: Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Belongs to the IRF family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; interferon type I biosynthetic process; defense response to virus |
| UniProt Code: | Q00978 |
| NCBI GenInfo Identifier: | 266392 |
| NCBI Gene ID: | 10379 |
| NCBI Accession: | Q00978.1 |
| UniProt Secondary Accession: | Q00978,D3DS61, |
| UniProt Related Accession: | Q00978 |
| Molecular Weight: | 393 |
| NCBI Full Name: | Interferon regulatory factor 9 |
| NCBI Synonym Full Names: | interferon regulatory factor 9 |
| NCBI Official Symbol: | IRF9 |
| NCBI Official Synonym Symbols: | p48; IRF-9; ISGF3; ISGF3G |
| NCBI Protein Information: | interferon regulatory factor 9; ISGF-3 gamma; ISGF3 p48 subunit; interferon-stimulated gene factor 3 gamma; transcriptional regulator ISGF3 subunit gamma; IFN-alpha-responsive transcription factor subunit; interferon-stimulated transcription factor 3, gamma 48kDa |
| UniProt Protein Name: | Interferon regulatory factor 9 |
| UniProt Synonym Protein Names: | IFN-alpha-responsive transcription factor subunit; ISGF3 p48 subunit; Interferon-stimulated gene factor 3 gamma; ISGF-3 gamma; Transcriptional regulator ISGF3 subunit gamma |
| Protein Family: | Interferon regulatory factor |
| UniProt Gene Name: | IRF9 |
| UniProt Entry Name: | IRF9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IRF9 antibody (CAB1004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
View AllClose
Related Products
IRF9 Polyclonal Antibody
OverviewProduct Name:IRF9 Polyclonal AntibodyProduct Code:CAB21331Reactivity:HumanApplications:Western blotting
