Immunology Antibodies 1
Anti-IRF5 Antibody (CAB1149)
- SKU:
- CAB1149
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-IRF5 Antibody |
| Antibody SKU: | CAB1149 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | THP-1, U-937, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human IRF5 (NP_001092097.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNQS IPVA PTPP RRVR LKPW LVAQ VNSC QYPG LQWV NGEK KLFC IPWR HATR HGPS QDGD NTIF KAWA KETG KYTE GVDE ADPA KWKA NLRC ALNK SRDF RLIY DGPR DMPP QPYK IYEV CSNG PAPT DSQP PEDY SFGA GEEE EEEE ELQR MLPS LSLT EDVK WPPT LQPP TLRP PTLQ PPTL QPPV VLGP PAPD PSPL |
| Gene ID: | 3663 |
| Uniprot: | Q13568 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 17kDa/47kDa/54kDa/56kDa/57kDa |
| Observed MW: | 56kDa/60kDa |
| Synonyms: | IRF5, SLEB10 |
| Background: | This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. |
| UniProt Protein Function: | IRF5: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. Homodimer, when phosphorylated. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 7q32 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: transcription, DNA-dependent; positive regulation of apoptosis; response to muramyl dipeptide; positive regulation of interleukin-12 production; cytokine and chemokine mediated signaling pathway; response to peptidoglycan; positive regulation of interferon-beta production; positive regulation of transcription from RNA polymerase II promoter; defense response to virus; positive regulation of interferon-alpha production Disease: Systemic Lupus Erythematosus, Susceptibility To, 10; Inflammatory Bowel Disease 14 |
| NCBI Summary: | This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010] |
| UniProt Code: | Q13568 |
| NCBI GenInfo Identifier: | 20178305 |
| NCBI Gene ID: | 3663 |
| NCBI Accession: | Q13568.2 |
| UniProt Secondary Accession: | Q13568,Q1A7B4, Q64GA9, Q64GB1, Q64GB2, Q6RCM8, Q9BQF0 A4D1J8, A8DUA8, A8DUA9, E7EQ16, E7EW54, |
| UniProt Related Accession: | Q13568 |
| Molecular Weight: | 498 |
| NCBI Full Name: | Interferon regulatory factor 5 |
| NCBI Synonym Full Names: | interferon regulatory factor 5 |
| NCBI Official Symbol: | IRF5 |
| NCBI Official Synonym Symbols: | SLEB10 |
| NCBI Protein Information: | interferon regulatory factor 5 |
| UniProt Protein Name: | Interferon regulatory factor 5 |
| Protein Family: | Interferon regulatory factor |
| UniProt Gene Name: | IRF5 |
| UniProt Entry Name: | IRF5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IRF5 antibody (CAB1149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using IRF5 antibody (CAB1149). Blue: DAPI for nuclear staining. |
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