Antibody Name:	Anti-IRF3 Antibody [KO Validated]
Antibody SKU:	CAB0816
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IRF3 (NP_001184051.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, Jurkat, HepG2, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IRF3 (NP_001184051.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PHDP HKIY EFVN SGVG DFSQ PDTS PDTN GGGS TSDT QEDI LDEL LGNM VLAP LPDP GPPS LAVA PEPC PQPL RSPS LDNP TPFP NLGP SENP LKRL LVPG E
Gene ID:	3661
Uniprot:	Q14653
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	12kDa/16kDa/33kDa/47kDa/49kDa
Observed MW:	47kDa.55kDa

Synonyms:

IIAE7, IRF3

Background:

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; negative regulation of interferon-beta biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus by host; response to exogenous dsRNA; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon-beta production; innate immune response; interferon type I biosynthetic process; defense response to virus; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; negative regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of cytokine secretion Disease: Herpes Simplex Encephalitis, Susceptibility To, 7
NCBI Summary:	This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	Q14653
NCBI GenInfo Identifier:	2497442
NCBI Gene ID:	3661
NCBI Accession:	Q14653.1
UniProt Secondary Accession:	Q14653,Q5FBY1, Q5FBY2, Q5FBY4, Q7Z5G6, A8K7L2, B2RAZ3
UniProt Related Accession:	Q14653
Molecular Weight:	12,330 Da
NCBI Full Name:	Interferon regulatory factor 3
NCBI Synonym Full Names:	interferon regulatory factor 3
NCBI Official Symbol:	IRF3
NCBI Protein Information:	interferon regulatory factor 3
UniProt Protein Name:	Interferon regulatory factor 3
Protein Family:	Interferon regulatory factor
UniProt Gene Name:	IRF3
UniProt Entry Name:	IRF3_HUMAN

	Western blot analysis of extracts of various cell lines, using IRF3 antibody (CAB0816) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and IRF3 Rabbit pAb knockout (KO) HeLa cells, using IRF3 Rabbit pAb antibody (CAB0816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human breast using IRF3 antibody (CAB0816) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using IRF3 antibody (CAB0816) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using IRF3 antibody (CAB0816) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-IRF3 Antibody (CAB11118)[KO Validated]	Human IRF3 ELISA Kit
Anti-IRF3 Antibody (CAB11373)	Human Interferon regulatory factor 3 (IRF3) ELISA Kit
Anti-IRF3 Antibody (CAB2172)	IRF-3 (Phospho-Ser396) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-IRF3-S396 Antibody (CABP0623)	IRF-3 (Phospho-Ser385) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-IRF3-S386 pAb Antibody (CABP0857)	IRF-3 (Phospho-Ser386) Colorimetric Cell-Based ELISA Kit