Name: Anti-IRAK4 Antibody (CAB6208)
Brand: Immunology Antibodies 2
SKU: CAB6208
Availability: OutOfStock

Antibody Name:	Anti-IRAK4 Antibody
Antibody SKU:	CAB6208
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNKP ITPS TYVR CLNV GLIR KLSD FIDP QEGW KKLA VAIK KPSG DDRY NQFH IRRF EALL QTGK SPTS ELLF DWGT TNCT VGDL VDLL IQNE FFAP ASLL LPDA VPKT ANTL PSKE AITV QQKQ MPFC DKDR TLMT PVQN LEQS YMPP DSSS PENK SLEV SDTR FHSF SFYE LKNV TNNF DERP ISVG GNKM GEGG FGVV YKGY VNNT TVAV KKLA AMVD
Gene ID:	51135
Uniprot:	Q9NWZ3
Cellular Location:	Cytoplasm
Calculated MW:	37kDa/51kDa
Observed MW:	55KDa

Synonyms:	IRAK4, IPD1, IRAK-4, NY-REN-64, REN64
Background:	This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.
UniProt Protein Details:	Protein type:EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; IRAK family Chromosomal Location of Human Ortholog: 12q12 Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; positive regulation of smooth muscle cell proliferation; cytokine production; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency
NCBI Summary:	This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q9NWZ3
NCBI GenInfo Identifier:	50401181
NCBI Gene ID:	51135
NCBI Accession:	Q9NWZ3.1
UniProt Secondary Accession:	Q9NWZ3,Q69FE1, Q8TDF7, Q9Y589,
UniProt Related Accession:	Q9NWZ3
Molecular Weight:	37,674 Da
NCBI Full Name:	Interleukin-1 receptor-associated kinase 4
NCBI Synonym Full Names:	interleukin-1 receptor-associated kinase 4
NCBI Official Symbol:	IRAK4
NCBI Official Synonym Symbols:	IPD1; REN64; IRAK-4; NY-REN-64
NCBI Protein Information:	interleukin-1 receptor-associated kinase 4; renal carcinoma antigen NY-REN-64
UniProt Protein Name:	Interleukin-1 receptor-associated kinase 4
UniProt Synonym Protein Names:	Renal carcinoma antigen NY-REN-64
Protein Family:	Interleukin-1 receptor-associated kinase
UniProt Gene Name:	IRAK4
UniProt Entry Name:	IRAK4_HUMAN

	Western blot analysis of extracts of various cell lines, using IRAK4 antibody (CAB6208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat spleen using IRAK4 Rabbit pAb (CAB6208) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using IRAK4 Rabbit pAb (CAB6208) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using IRAK4 Rabbit pAb (CAB6208) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using IRAK4 Rabbit pAb (CAB6208) at dilution of 1:50 (40x lens).