Name: Anti-IRAK2 Antibody (CAB6635)
Brand: Cell Biology Antibodies 10
SKU: CAB6635
Availability: OutOfStock

Antibody Name:	Anti-IRAK2 Antibody
Antibody SKU:	CAB6635
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human IRAK2 (NP_001561.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, HepG2, NCI-H460, Mouse liver, Mouse kidney, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human IRAK2 (NP_001561.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MACY IYQL PSWV LDDL CRNM DALS EWDW MEFA SYVI TDLT QLRK IKSM ERVQ GVSI TREL LWWW GMRQ ATVQ QLVD LLCR LELY RAAQ IILN WKPA PEIR CPIP AFPD SVKP EKPL AASV RKAE DEQE EGQP VRMA TFPG PGSS PARA HQPA FLQP PEED APHS LRSD LPTS SDSK DFST SIPK QEKL LSLA GDSL FWSE ADVV QATD DFNQ NRKI SQGT FADV YRGH RHGK PFVF KKLR ETAC SSPG SIER FFQA ELQI CLRC CHPN VL
Gene ID:	3656
Uniprot:	O43187
Cellular Location:
Calculated MW:	69kDa
Observed MW:	75kDa

Synonyms:	IRAK2, IRAK-2
Background:	IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB.

UniProt Protein Function:	IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; IRAK family Chromosomal Location of Human Ortholog: 3p25.3 Cellular Component: cytoplasm; cytosol; endosome membrane; nucleus; plasma membrane Molecular Function:protein binding; protein heterodimerization activity; protein homodimerization activity; protein serine/threonine kinase activity Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; I-kappaB kinase/NF-kappaB cascade; inflammatory response; inhibition of NF-kappaB transcription factor; JNK cascade; MyD88-dependent toll-like receptor signaling pathway; protein amino acid phosphorylation; regulation of cytokine and chemokine mediated signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway
NCBI Summary:	IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. [provided by RefSeq, Jul 2008]
UniProt Code:	O43187
NCBI GenInfo Identifier:	125987833
NCBI Gene ID:	3656
NCBI Accession:	O43187.2
UniProt Secondary Accession:	O43187,Q08AG6, Q5K546, B4DQZ6,
UniProt Related Accession:	O43187
Molecular Weight:	69,433 Da
NCBI Full Name:	Interleukin-1 receptor-associated kinase-like 2
NCBI Synonym Full Names:	interleukin 1 receptor associated kinase 2
NCBI Official Symbol:	IRAK2
NCBI Official Synonym Symbols:	IRAK-2
NCBI Protein Information:	interleukin-1 receptor-associated kinase-like 2
UniProt Protein Name:	Interleukin-1 receptor-associated kinase-like 2
Protein Family:	Interleukin-1 receptor-associated kinase
UniProt Gene Name:	IRAK2
UniProt Entry Name:	IRAK2_HUMAN

	Western blot analysis of extracts of various cell lines, using IRAK2 antibody (CAB6635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using IRAK2 antibody (CAB6635). Blue: DAPI for nuclear staining.