Immunology Antibodies 1
Anti-IRAK1 Antibody (CAB12624)
- SKU:
- CAB12624
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-IRAK1 Antibody |
| Antibody SKU: | CAB12624 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 424-633 of human IRAK1 (NP_001020414.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:3000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-549, Mouse liver, Mouse kidney, Mouse skeletal muscle, Rat liver, Rat kidney, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 424-633 of human IRAK1 (NP_001020414.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VKTH GART KYLV YERL EKLQ AVVA GVPG HSEA ASCI PPSP QENS YVSS TGRA HSGA APWQ PLAA PSGA SAQA AEQL QRGP NQPV ESDE SLGG LSAA LRSW HLTP SCPL DPAP LREA GCPQ GDTA GESS WGSG PGSR PTAV EGLA LGSS ASSS SEPP QIII NPAR QKMV QKLA LYED GALD SLQL LSSS SLPG LGLE QDRQ GPEE SDEF QS |
| Gene ID: | 3654 |
| Uniprot: | P51617 |
| Cellular Location: | Cytoplasm, Lipid droplet, Nucleus |
| Calculated MW: | 68kDa/73kDa/74kDa/76kDa |
| Observed MW: | 90-100kDa |
| Synonyms: | IRAK1, IRAK, pelle |
| Background: | This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. |
| UniProt Protein Details: | Protein type:Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; IRAK family Chromosomal Location of Human Ortholog: Xq28 Cellular Component: cytoplasm; cytosol; endosome membrane; lipid particle; nucleus; plasma membrane Molecular Function:kinase activity; NF-kappaB-inducing kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; inhibition of NF-kappaB transcription factor; JNK cascade; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of MAP kinase activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein oligomerization; regulation of cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway |
| NCBI Summary: | This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P51617 |
| NCBI GenInfo Identifier: | 8928535 |
| NCBI Gene ID: | 3654 |
| NCBI Accession: | P51617.2 |
| UniProt Secondary Accession: | P51617,Q7Z5V4, Q96C06, Q96RL2, D3DWW3, D3DWW4, |
| UniProt Related Accession: | P51617 |
| Molecular Weight: | 68,022 Da |
| NCBI Full Name: | Interleukin-1 receptor-associated kinase 1 |
| NCBI Synonym Full Names: | interleukin 1 receptor associated kinase 1 |
| NCBI Official Symbol: | IRAK1 |
| NCBI Official Synonym Symbols: | IRAK; pelle |
| NCBI Protein Information: | interleukin-1 receptor-associated kinase 1 |
| UniProt Protein Name: | Interleukin-1 receptor-associated kinase 1 |
| Protein Family: | Interleukin-1 receptor-associated kinase |
| UniProt Gene Name: | IRAK1 |
| UniProt Entry Name: | IRAK1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IRAK1 antibody (CAB12624) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of C6 cells using IRAK1 antibody (CAB12624) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using IRAK1 antibody (CAB12624) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using IRAK1 antibody (CAB12624) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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