KO Validated Antibodies 2

Anti-IPO9 Antibody (CAB19860)[KO Validated]

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SKU:
CAB19860
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-IPO9 Antibody [KO Validated]
Antibody SKU:CAB19860
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human IPO9.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human IPO9.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:55705
Uniprot:Q96P70
Cellular Location:Cytoplasm, Nucleus
Calculated MW:115kDa
Observed MW:130KDa
Anti-IPO9 Antibody [KO Validated] (CAB19860)Western blot analysis of extracts from normal (control) and IPO9 knockout (KO) HeLa cells, using IPO9 antibody (CAB19860) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Synonyms:IPO9, Imp9
Background:
UniProt Protein Function:IPO9: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of H2B histone, RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones, RPL4 and RPL6. Belongs to the importin beta family.
UniProt Protein Details:

Protein type:Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytoplasm; membrane

Molecular Function:histone binding; protein binding; protein transporter activity

UniProt Code:Q96P70
NCBI GenInfo Identifier:41688593
NCBI Gene ID:55705
NCBI Accession:Q96P70.3
UniProt Secondary Accession:Q96P70,Q8N1Y1, Q8N3I2, Q8NCG9, Q96SU6, Q9NW01, Q9P0A8 Q9ULM8, B1ASV5,
UniProt Related Accession:Q96P70
Molecular Weight:116kDa
NCBI Full Name:Importin-9
NCBI Synonym Full Names:importin 9
NCBI Official Symbol:IPO9  
NCBI Official Synonym Symbols:Imp9  
NCBI Protein Information:importin-9
UniProt Protein Name:Importin-9
UniProt Synonym Protein Names:Ran-binding protein 9; RanBP9
Protein Family:Importin
UniProt Gene Name:IPO9  
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