Antibody Name:	Anti-IPO5 Antibody
Antibody SKU:	CAB1984
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human IPO5 (NP_002262.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, HeLa, 293T, HepG2, Mouse testis, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human IPO5 (NP_002262.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAAA AEQQ QFYL LLGN LLSP DNVV RKQA EETY ENIP GQSK ITFL LQAI RNTT AAEE ARQM AAVL LRRL LSSA FDEV YPAL PSDV QTAI KSEL LMII QMET QSSM RKKV CDIA AELA RNLI DEDG NNQW PEGL KFLF DSVS SQNV GLRE AALH IFWN FPGI FGNQ QQHY LDVI KRML VQCM QDQE HPSI RTLS ARAT AAFI LANE HNVA LFKH FADL LPGF LQAV NDSC YQND DSVL KSLV EIAD TVPK YLRP HLEA TLQL SLKL CGDT SLNN MQRQ LALE V
Gene ID:	3843
Uniprot:	O00410
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	116kDa/123kDa/125kDa
Observed MW:	124kDa

Synonyms:

IPO5, IMB3, KPNB3, Pse1, RANBP5, imp5

Background:

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

UniProt Protein Function:	KPNB3: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Belongs to the importin beta family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Karyopherin; Nucleolus; Nuclear import Chromosomal Location of Human Ortholog: 13q32.2 Cellular Component: nucleoplasm; Golgi apparatus; nuclear membrane; intracellular membrane-bound organelle; membrane; cytoplasm; nucleolus; nuclear pore; nucleus Molecular Function:protein binding; Ran GTPase binding; protein transporter activity; GTPase inhibitor activity Biological Process: ribosomal protein import into nucleus; positive regulation of protein import into nucleus; viral reproduction; negative regulation of catalytic activity; NLS-bearing substrate import into nucleus
NCBI Summary:	Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008]
UniProt Code:	O00410
NCBI GenInfo Identifier:	4033763
NCBI Gene ID:	3843
NCBI Accession:	O00410.4
UniProt Related Accession:	O00410
Molecular Weight:	Predicted Molecular Mass: 28.9kDaAccurate Molecular Mass: 26kDa
NCBI Full Name:	Importin-5
NCBI Synonym Full Names:	importin 5
NCBI Official Symbol:	IPO5
NCBI Official Synonym Symbols:	IMB3; Pse1; imp5; KPNB3; RANBP5
NCBI Protein Information:	importin-5
UniProt Protein Name:	Importin-5
UniProt Synonym Protein Names:	Importin subunit beta-3; Karyopherin beta-3; Ran-binding protein 5; RanBP5
Protein Family:	Importin
UniProt Gene Name:	IPO5
UniProt Entry Name:	IPO5_HUMAN

	Western blot analysis of extracts of various cell lines, using IPO5 antibody (CAB1984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of U2OS cells using IPO5 antibody (CAB1984). Blue: DAPI for nuclear staining.