KO Validated Antibodies 2

Anti-IPO4 Antibody (CAB19902)[KO Validated]

(No reviews yet) Write a Review
SKU:
CAB19902
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-IPO4 Antibody [KO Validated]
Antibody SKU:CAB19902
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human IPO4.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human IPO4.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:79711
Uniprot:Q8TEX9
Cellular Location:Cytoplasm, Nucleus
Calculated MW:119kDa
Observed MW:130KDa
Anti-IPO4 Antibody [KO Validated] (CAB19902)Western blot analysis of extracts from normal (control) and IPO4 knockout (KO) 293T cells, using IPO4 antibody (CAB19902) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:IPO4, Imp4
Background:
UniProt Protein Function:IPO4: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: protein complex; membrane; nuclear chromatin; cytoplasm; nuclear pore

Molecular Function:protein binding; Ran GTPase binding

Biological Process: intracellular protein transport; protein transport; DNA replication-independent nucleosome assembly; DNA replication-dependent nucleosome assembly

UniProt Code:Q8TEX9
NCBI GenInfo Identifier:62460637
NCBI Gene ID:79711
NCBI Accession:NP_078934.3
UniProt Secondary Accession:Q8TEX9,Q2NL96, Q86TZ9, Q8NCG8, Q96SJ3, Q9BTI4, Q9H5L0 B2RN95,
UniProt Related Accession:Q8TEX9
Molecular Weight:118,715 Da
NCBI Full Name:importin-4
NCBI Synonym Full Names:importin 4
NCBI Official Symbol:IPO4  
NCBI Official Synonym Symbols:Imp4  
NCBI Protein Information:importin-4; imp4b; ranBP4; importin-4b; ran-binding protein 4
UniProt Protein Name:Importin-4
UniProt Synonym Protein Names:Importin-4b; Imp4b; Ran-binding protein 4; RanBP4
Protein Family:Importin
UniProt Gene Name:IPO4  
UniProt Entry Name:IPO4_HUMAN
View AllClose

0 Reviews

View AllClose

Related Products