Cell Biology Antibodies 5

Anti-IPO11 Antibody (CAB14680)

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SKU:
CAB14680
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-IPO11 Antibody
Antibody SKU:CAB14680
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 776-975 of human IPO11 (NP_057422.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, 293T, A-549, Mouse heart, Mouse kidney, Mouse lung, Mouse spleen, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 776-975 of human IPO11 (NP_057422.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KGII EGER YPVV MSTY LGVM GRVL LQNT SFFS SLLN EMAH KFNQ EMDQ LLGN MIEM WVDR MDNI TQPE RRKL SALA LLSL LPSD NSVI QDKF CGII NISV EGLH DVMT EDPE TGTY KDCM LMSH LEEP KVTE DEEP PTEQ DKRK KMLA LKDP VHTV SLQQ FIYE KLKA QQEM LGEQ GFQS LMET VDTE IVTQ LQEF LQGF
Gene ID:51194
Uniprot:Q9UI26
Cellular Location:Cytoplasm, Nucleus
Calculated MW:112kDa/117kDa
Observed MW:112kDa
Synonyms:IPO11, RanBP11
Background:
UniProt Protein Function:IPO11: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of UBE2E3, and of RPL12. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: cytosol; nuclear envelope

Molecular Function:protein binding; protein transporter activity

Biological Process: transcription factor import into nucleus

NCBI Summary:Importins, including IPO11, are a members of the karyopherin/importin-beta family of transport receptors (see KPNB1; 602738) that mediate nucleocytoplasmic transport of protein and RNA cargoes (Plafker and Macara, 2000 [PubMed 11032817]).[supplied by OMIM, Sep 2008]
UniProt Code:Q9UI26
NCBI GenInfo Identifier:50401199
NCBI Gene ID:51194
NCBI Accession:Q9UI26.1
UniProt Secondary Accession:Q9UI26,Q8N5R2, Q9NSJ6, Q9NVB1, A6NGJ5, B4DZ73, D3DW98
UniProt Related Accession:Q9UI26
Molecular Weight:117,008 Da
NCBI Full Name:Importin-11
NCBI Synonym Full Names:importin 11
NCBI Official Symbol:IPO11  
NCBI Official Synonym Symbols:RanBP11  
NCBI Protein Information:importin-11
UniProt Protein Name:Importin-11
UniProt Synonym Protein Names:Ran-binding protein 11; RanBP11
Protein Family:Importin
UniProt Gene Name:IPO11  
UniProt Entry Name:IPO11_HUMAN
Anti-IPO11 Antibody (CAB14680)Western blot analysis of extracts of various cell lines, using IPO11 antibody (CAB14680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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