Antibody Name:	Anti-IP3R Antibody
Antibody SKU:	CAB7905
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1850-1950 of human IP3R (XP_011531985.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HT-1080, Mouse kidney, Mouse brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1850-1950 of human IP3R (XP_011531985.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SFFC RLTE DKKS EKFF KVFY DRMK VAQQ EIKA TVTV NTSD LGNK KKDD EVDR DAPS RKKA KEPT TQIT EEVR DQLL EASA ATRK AFTT FRRE ADPD DHYQ P
Gene ID:	3708
Uniprot:	Q14643
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	306-313kDa
Observed MW:	315kDa

Synonyms:

ITPR1, ACV, CLA4, INSP3R1, IP3R, IP3R1, PPP1R94, SCA15, SCA16, SCA29

Background:

This gene encodes an intracellular receptor for inositol 1, 4, 5-trisphosphate. Upon stimulation by inositol 1, 4, 5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.

UniProt Protein Function:	IP3R1: a multi-pass endoplasmic reticulum membrane receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaMKII, eventually leading to the activation of downstream apoptosis pathways. Part of a cGMP kinase signaling complex that includes alpha-actin, calponin H1, phospholamban, PKG1 and IP3R1. Widely expressed. The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. Phosphorylation by PKA prevents the ligand-induced opening of the calcium channels. Belongs to the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family. Eight alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p26.1 Cellular Component: calcineurin complex; endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane; nuclear inner membrane; nucleolus; platelet dense granule membrane; postsynaptic density; sarcoplasmic reticulum Molecular Function:calcium channel inhibitor activity; calcium ion transmembrane transporter activity; inositol 1,4,5-triphosphate-sensitive calcium-release channel activity; intracellular ligand-gated calcium channel activity; phosphoinositide binding; protein binding Biological Process: blood coagulation; calcium ion transport; endoplasmic reticulum calcium ion homeostasis; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; epithelial fluid transport; fibroblast growth factor receptor signaling pathway; innate immune response; inositol phosphate-mediated signaling; negative regulation of calcium-mediated signaling; nerve growth factor receptor signaling pathway; phospholipase C activation; platelet activation; post-embryonic development; regulation of insulin secretion; release of sequestered calcium ion into cytosol; response to hypoxia; signal transduction; stimulatory C-type lectin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; voluntary musculoskeletal movement
NCBI Summary:	This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]
UniProt Code:	Q14643
NCBI GenInfo Identifier:	519668682
NCBI Gene ID:	3708
NCBI Accession:	Q14643.3
UniProt Secondary Accession:	Q14643,Q14660, Q99897, E7EPX7, E9PDE9,
UniProt Related Accession:	Q14643
Molecular Weight:
NCBI Full Name:	Inositol 1,4,5-trisphosphate receptor type 1
NCBI Synonym Full Names:	inositol 1,4,5-trisphosphate receptor type 1
NCBI Official Symbol:	ITPR1
NCBI Official Synonym Symbols:	ACV; CLA4; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1; PPP1R94
NCBI Protein Information:	inositol 1,4,5-trisphosphate receptor type 1
UniProt Protein Name:	Inositol 1,4,5-trisphosphate receptor type 1
UniProt Synonym Protein Names:	IP3 receptor isoform 1; IP3R 1; InsP3R1; Type 1 inositol 1,4,5-trisphosphate receptor; Type 1 InsP3 receptor
Protein Family:	Inositol 1,4,5-trisphosphate receptor
UniProt Gene Name:	ITPR1
UniProt Entry Name:	ITPR1_HUMAN

	Western blot analysis of extracts of various cell lines, using IP3R antibody (CAB7905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U-2 OS cells using IP3R antibody (CAB7905) at dilution of 1:100. Blue: DAPI for nuclear staining.