Epigenetics & Nuclear Signaling Antibodies 4
Anti-Interleukin-33 Antibody (CAB8096)
- SKU:
- CAB8096
Description
| Antibody Name: | IL33 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8096 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 95-270 of human IL33 (NP_254274.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, SGC-7901, MCF7, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 95-270 of human IL33 (NP_254274.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AFGI SGVQ KYTR ALHD SSIT GISP ITEY LASL STYN DQSI TFAL EDES YEIY VEDL KKDE KKDK VLLS YYES QHPS NESG DGVD GKML MVTL SPTK DFWL HANN KEHS VELH KCEK PLPD QAFF VLHN MHSN CVSF ECKT DPGV FIGV KDNH LALI KVDS SENL CTEN ILFK LSET |
| Gene ID: | 90865 |
| Uniprot: | O95760 |
| Cellular Location: | Chromosome, Cytoplasmic vesicle, Nucleus, Secreted, secretory vesicle |
| Calculated MW: | 16kDa/25kDa/26kDa/30kDa |
| Observed MW: | 35kDa |
| Synonyms: | IL33, C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV |
| Background: | The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine Chromosomal Location of Human Ortholog: 9p24.1 Cellular Component: extracellular space; transport vesicle; chromosome; nucleus Molecular Function:protein binding; cytokine activity Biological Process: T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; positive regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response |
| NCBI Summary: | IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011] |
| UniProt Code: | O95760 |
| NCBI GenInfo Identifier: | 68565855 |
| NCBI Gene ID: | 90865 |
| NCBI Accession: | O95760.1 |
| UniProt Secondary Accession: | O95760,Q2YEJ5, B2R8L1, B4DJ35, B4E1Q9, D3DRI5, E7EAX4 |
| UniProt Related Accession: | O95760 |
| Molecular Weight: | 16,250 Da |
| NCBI Full Name: | Interleukin-33 |
| NCBI Synonym Full Names: | interleukin 33 |
| NCBI Official Symbol: | IL33 |
| NCBI Official Synonym Symbols: | DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26 |
| NCBI Protein Information: | interleukin-33; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules |
| UniProt Protein Name: | Interleukin-33 |
| UniProt Synonym Protein Names: | Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL33 |
| UniProt Entry Name: | IL33_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IL33 antibody (CAB8096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using IL33 antibody (CAB8096) at dilution of 1:100 (40x lens). |
Additional Information
Product Type: |
Antibody |
Applications: |
WB |
Applications: |
IHC |
Reactivity: |
Human |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
Research Area: |
Epigenetics and Nuclear Signaling |
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