Cell Biology Antibodies 12
Anti-Interleukin-25 Antibody (CAB8252)
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- SKU:
- CAB8252
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | IL25 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8252 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-937, MCF7, BT-474, HepG2, Mouse liver, Mouse heart, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YSHW PSCC PSKG QDTS EELL RWST VPVP PLEP ARPN RHPE SCRA SEDG PLNS RAIS PWRY ELDR DLNR LPQD LYHA RCLC PHCV SLQT GSHM DPRG NSEL LYHN QTVF YRRP CHGE KGTH KGYC LERR LYRV SLAC VCVR PRVM G |
| Gene ID: | 64806 |
| Uniprot: | Q9H293 |
| Cellular Location: | Secreted |
| Calculated MW: | 18kDa/20kDa |
| Observed MW: | 24kDa |
| Synonyms: | IL25, IL17E |
| Background: | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: extracellular space Molecular Function:cytokine activity; interleukin-17E receptor binding Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| UniProt Code: | Q9H293 |
| NCBI GenInfo Identifier: | 20138730 |
| NCBI Gene ID: | 64806 |
| NCBI Accession: | Q9H293.1 |
| UniProt Secondary Accession: | Q9H293,Q2M3F0, Q8IZV3, Q8WXB0, |
| UniProt Related Accession: | Q9H293 |
| Molecular Weight: | 18,523 Da |
| NCBI Full Name: | Interleukin-25 |
| NCBI Synonym Full Names: | interleukin 25 |
| NCBI Official Symbol: | IL25 |
| NCBI Official Synonym Symbols: | IL17E |
| NCBI Protein Information: | interleukin-25; interleukin-17E |
| UniProt Protein Name: | Interleukin-25 |
| UniProt Synonym Protein Names: | Interleukin-17E; IL-17E |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL25 |
| UniProt Entry Name: | IL25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IL25 antibody (CAB8252) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat lung using IL25 antibody (CAB8252) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (CAB8252) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (CAB8252) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of human lung cancer cells using IL25 antibody (CAB8252) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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