Antibody Name:	Anti-Integrin alpha 6 (ITGA6/CD49f) Antibody (CAB20807)
Antibody SKU:	CAB20807
Antibody Size:	50µL, 100µL
Application:	Western blotting
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 481-591 of human Integrin alpha 6 (ITGA6/CD49f) (NP_001073286.1).

Application:	Western blotting
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	PC-3, U-251MG, Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 481-591 of human Integrin alpha 6 (ITGA6/CD49f) (NP_001073286.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IDLR QKTA CGAP SGIC LQVK SCFE YTAN PAGY NPSI SIVG TLEA EKER RKSG LSSR VQFR NQGS EPKY TQEL TLKR QKQK VCME ETLW LQDN IRDK LRPI PITA SVEI QEP
Cellular Location:	Cell membrane, Lipid-anchor, Single-pass type I membrane protein
Calculated MW:	106kDa/119kDa/121kDa/122kDa/124kDa/126kDa
Observed MW:	125KDa

Synonyms:

CD49f, ITGA6B, VLA-6, Integrin alpha 6, ITGA6

Background:

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants.

	Western blot analysis of extracts of various cell lines, using Integrin alpha 6 (ITGA6/CD49f) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
	Western blot analysis of extracts of various cell lines, using Integrin alpha 6 (ITGA6/CD49f) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.