Metabolism Antibodies 2

Anti-INSIG2 Antibody (CAB1992)

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SKU:
CAB1992
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-INSIG2 Antibody
Antibody SKU:CAB1992
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human INSIG2 (NP_057217.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, Mouse kidney, Mouse spleen, Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human INSIG2 (NP_057217.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QIQR NVTL FPPD VIAS IFSS AWWV PPCC GTAS AVIG LLYP CIDR HLGE PHKF KREW SSVM RCVA VFVG INHA SAKV DFDN NIQL SLTL AALS IGLW WTFD R
Gene ID:51141
Uniprot:Q9Y5U4
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:24kDa
Observed MW:27kDa
Synonyms:INSIG2, INSIG-2
Background:The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.
UniProt Protein Function:Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR.
NCBI Summary:The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y5U4
NCBI GenInfo Identifier:1008806542
NCBI Gene ID:51141
NCBI Accession:NP_001308258.1
UniProt Secondary Accession:Q9Y5U4,Q8TBI8, A8K5W8,
UniProt Related Accession:Q9Y5U4
Molecular Weight:24
NCBI Full Name:insulin-induced gene 2 protein isoform a
NCBI Synonym Full Names:insulin induced gene 2
NCBI Official Symbol:INSIG2  
NCBI Official Synonym Symbols:INSIG-2  
NCBI Protein Information:insulin-induced gene 2 protein
UniProt Protein Name:Insulin-induced gene 2 protein
UniProt Gene Name:INSIG2  
Anti-INSIG2 Antibody (CAB1992)Western blot analysis of extracts of various cell lines, using INSIG2 antibody (CAB1992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1.
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