Cell Death Antibodies 1

Anti-INPP5D Antibody (CAB0122)

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SKU:
CAB0122
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-INPP5D Antibody
Antibody SKU:CAB0122
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human INPP5D (NP_005532.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human INPP5D (NP_005532.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPPT PPGQ PPIS PKKF LPST ANRG LPPR TQES RPSD LGKN AGDT LPQE DLPL TKPE MFEN PLYG SLSS FPKP APRK DQES PKMP RKEP PPCP EPGI LSPS IVLT KAQE ADRG EGPG KQVP APRL RSFT CSSS AEGR AAGG DKSQ GKPK TPVS SQAP VPAK RPIK PSRS EINQ QTPP TPTP RPPL PVKS PAVL HLQH SKGR DYRD NTEL PHHG KHRP EEGP PGPL GRTA MQ
Gene ID:3635
Uniprot:Q92835
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:109kDa/133kDa
Observed MW:150kDa
Synonyms:INPP5D, SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship
Background:This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3, 4, 5)-trisphosphate and inositol-1, 3, 4, 5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants.
UniProt Protein Function:SHIP: an SH2-containing inositol phosphatase. A hemopoietic-specific phosphatase that regulates cell survival, growth, cell cycle arrest and apoptosis. Hydrolyzes Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3. A cytosolic protein with a SH2 domain in its N-terminus and two NPXY Shc binding motifs at its C-terminus. Upon receptor cross-linking, SHIP is first recruited to the plasma membrane by its SH2, followed by tyrosine phosphorylation on the NPXY motif. Both membrane relocalization and phosphorylation are essential for its regulatory function. Associates with SHC after cytokine stimulation.
UniProt Protein Details:

Protein type:Phosphatase, lipid; EC 3.1.3.86; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytoskeleton; cytosol; lipid raft; plasma membrane

Molecular Function:inositol-polyphosphate 5-phosphatase activity; protein binding; PTB domain binding; SH3 domain binding

Biological Process: apoptosis; blood coagulation; determination of adult life span; immunoglobulin mediated immune response; inositol phosphate metabolic process; leukocyte migration; negative regulation of B cell proliferation; negative regulation of bone resorption; negative regulation of immune response; negative regulation of interleukin-6 biosynthetic process; negative regulation of monocyte differentiation; negative regulation of neutrophil differentiation; negative regulation of osteoclast differentiation; negative regulation of signal transduction; phosphate metabolic process; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation; phospholipid metabolic process; positive regulation of apoptosis; positive regulation of B cell differentiation; positive regulation of erythrocyte differentiation; signal transduction; T cell receptor signaling pathway

NCBI Summary:This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:Q92835
NCBI GenInfo Identifier:158564077
NCBI Gene ID:3635
NCBI Accession:Q92835.2
UniProt Secondary Accession:Q92835,O00145, Q13544, Q13545, Q6P5A4, Q92656, Q9UE80
UniProt Related Accession:Q92835
Molecular Weight:109,294 Da
NCBI Full Name:Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
NCBI Synonym Full Names:inositol polyphosphate-5-phosphatase D
NCBI Official Symbol:INPP5D  
NCBI Official Synonym Symbols:SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship  
NCBI Protein Information:phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
UniProt Protein Name:Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
UniProt Synonym Protein Names:Inositol polyphosphate-5-phosphatase of 145 kDa; SIP-145; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; SHIP-1; p150Ship; hp51CN
Protein Family:Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
UniProt Gene Name:INPP5D  
UniProt Entry Name:SHIP1_HUMAN
Anti-INPP5D Antibody (CAB0122)Western blot analysis of extracts of Jurkat cells, using INPP5D antibody (CAB0122). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-INPP5D Antibody (CAB0122)Immunofluorescence analysis of NIH/3T3 cells using INPP5D antibody (CAB0122) at dilution of 1:100. Blue: DAPI for nuclear staining.
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