Cell Biology Antibodies 8
Anti-iNOS Antibody (CAB3200)
- SKU:
- CAB3200
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-iNOS Antibody |
| Antibody SKU: | CAB3200 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human iNOS |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | RAW264.7 |
| Immunogen: | Recombinant protein of human iNOS |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4843 |
| Uniprot: | P35228 |
| Cellular Location: | |
| Calculated MW: | 126kDa/131kDa |
| Observed MW: | 131kDa |
| Synonyms: | NOS, INOS, NOS2A, HEP-NOS, iNOS, NOS2 |
| Background: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. |
| UniProt Protein Function: | Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. |
| UniProt Protein Details: | Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group By similarity.Binds 1 FAD By similarity.Binds 1 FMN By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity. Enzyme regulation: Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity By similarity. Subunit structure: Homodimer. Binds SLC9A3R1. Tissue specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Induction: By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Ref.14 Polymorphism: Note=Genetic variations in NOS2 are involved in resistance to malaria [ MIM:611162]. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain. |
| NCBI Summary: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35228 |
| NCBI GenInfo Identifier: | 1352513 |
| NCBI Gene ID: | 4843 |
| NCBI Accession: | P35228.2 |
| UniProt Secondary Accession: | P35228,O60757, O94994, Q16263, Q16692, Q4TTS5, Q9UD42 A1L3U5, B7ZLY2, |
| UniProt Related Accession: | P35228 |
| Molecular Weight: | 131,117 Da |
| NCBI Full Name: | Nitric oxide synthase, inducible |
| NCBI Synonym Full Names: | nitric oxide synthase 2, inducible |
| NCBI Official Symbol: | NOS2 |
| NCBI Official Synonym Symbols: | NOS; INOS; NOS2A; HEP-NOS |
| NCBI Protein Information: | nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes) |
| UniProt Protein Name: | Nitric oxide synthase, inducible |
| UniProt Synonym Protein Names: | Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2 |
| Protein Family: | Inositol 3-kinase |
| UniProt Gene Name: | NOS2 |
| UniProt Entry Name: | NOS2_HUMAN |
 | Western blot analysis of extracts of RAW264.7 cells, using iNOS antibody (CAB3200) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 ug/ml) at 37'C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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