Name: Anti-INMT Antibody (CAB14391)
Brand: Cell Biology Antibodies 5
SKU: CAB14391
Availability: OutOfStock

Antibody Name:	Anti-INMT Antibody
Antibody SKU:	CAB14391
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human INMT (NP_006765.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human INMT (NP_006765.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKGG FTGG DEYQ KHFL PRDY LATY YSFD GSPS PEAE MLKF NLEC LHKT FGPG GLQG DTLI DIGS GPTI YQVL AACD SFQD ITLS DFTD RNRE ELEK WLKK EPGA YDWT PAVK FACE LEGN SGRW EEKE EKLR AAVK RVLK CDVH LGNP LAPA VLPL ADCV LTLL AMEC ACCS LDAY RAAL CNLA SLLK PGGH LVTT VTLR LPSY MVGK REFS CVAL EKEE VEQA VLDA GFDI EQLL HSPQ SYSV TNAA NNGV CFIV ARKK PGP
Gene ID:	11185
Uniprot:	O95050
Cellular Location:	Cytoplasm
Calculated MW:	28kDa
Observed MW:	29kDa

Synonyms:	INMT, TEMT
Background:	N-methylation of endogenous and xenobiotic compounds is a major method by which they are degraded. This gene encodes an enzyme that N-methylates indoles such as tryptamine. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream FAM188B (family with sequence similarity 188, member B) gene.

UniProt Protein Function:	INMT: Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2- methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds. Catalyzes the N-methylation of tryptamine and structurally related compounds. Belongs to the NNMT/PNMT/TEMT family.
UniProt Protein Details:	Protein type:EC 2.1.1.96; Methyltransferase; EC 2.1.1.49; Amino Acid Metabolism - tryptophan Chromosomal Location of Human Ortholog: 7p14.3 Cellular Component: cytosol Molecular Function:amine N-methyltransferase activity; protein binding; thioether S-methyltransferase activity Biological Process: amine metabolic process; methylation; response to toxin; selenium metabolic process
NCBI Summary:	N-methylation of endogenous and xenobiotic compounds is a major method by which they are degraded. This gene encodes an enzyme that N-methylates indoles such as tryptamine. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream FAM188B (family with sequence similarity 188, member B) gene. [provided by RefSeq, Nov 2010]
UniProt Code:	O95050
NCBI GenInfo Identifier:	312836856
NCBI Gene ID:	11185
NCBI Accession:	NP_001186148.1
UniProt Secondary Accession:	O95050,Q3KP49, Q9P1Y2, Q9UBY4, Q9UHQ0, B8ZZ69,
UniProt Related Accession:	O95050
Molecular Weight:	44.9 kDa
NCBI Full Name:	indolethylamine N-methyltransferase isoform 2
NCBI Synonym Full Names:	indolethylamine N-methyltransferase
NCBI Official Symbol:	INMT
NCBI Official Synonym Symbols:	TEMT
NCBI Protein Information:	indolethylamine N-methyltransferase
UniProt Protein Name:	Indolethylamine N-methyltransferase
UniProt Synonym Protein Names:	Aromatic alkylamine N-methyltransferase; Amine N-methyltransferase; Arylamine N-methyltransferase; Thioether S-methyltransferase; TEMT
Protein Family:	Indolethylamine N-methyltransferase
UniProt Gene Name:	INMT
UniProt Entry Name:	INMT_HUMAN

	Western blot analysis of extracts of various cell lines, using INMT Rabbit pAb (CAB14391) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using INMT antibody (CAB14391) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using INMT antibody (CAB14391) at dilution of 1:100. Blue: DAPI for nuclear staining.